中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Establishment and characterization of a cell line from the head kidney of golden pompano Trachinotus ovatus and its application in toxicology and virus susceptibility

文献类型:期刊论文

作者Li, P; Zhou, L; Wei, S; Yang, M; Ni, S; Yu, Y; Cai, J; Qin, Q
刊名JOURNAL OF FISH BIOLOGY
出版日期2017
卷号90期号:5页码:1944-1959
关键词cytotoxicity head-kidney cell line Trachinotus ovatus transfection efficiency viral susceptibility
通讯作者qinqw@scsio.ac.cn
中文摘要A cell line derived from the head kidney of golden pompano Trachinotus ovatus (TOHK) was established and characterized in this study. The TOHK cells grew most rapidly at 28 degrees C and the optimum foetal bovine serum concentration in L-15 medium was 10%. The TOHK cells have a diploid chromosome number of 2N = 54. The transfection efficiency of TOHK cells was 75% at the 15th passage and 72% at the 40th passage. The transfection efficiency in TOHK cells was high, so these cells are suitable for foreign gene expression. The cytotoxic effects of heavy metals and extracellular products from Vibrio anguillarum and Vibrio alginolyticus were demonstrated in TOHK cells, so this TOHK cell line could also be applied in environmental monitoring of heavy metals and pathogenic bacteria. TOHK cell line showed high virus susceptibility, such as grouper nervous necrosis virus (GNNV) and Singapore grouper iridovirus (SGIV). Then, TOHK cell line could be used for the study of viral pathogenesis and the development of antiviral strategies.
源URL[http://ir.scsio.ac.cn/handle/344004/16278]  
专题南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
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GB/T 7714
Li, P,Zhou, L,Wei, S,et al. Establishment and characterization of a cell line from the head kidney of golden pompano Trachinotus ovatus and its application in toxicology and virus susceptibility[J]. JOURNAL OF FISH BIOLOGY,2017,90(5):1944-1959.
APA Li, P.,Zhou, L.,Wei, S.,Yang, M.,Ni, S.,...&Qin, Q.(2017).Establishment and characterization of a cell line from the head kidney of golden pompano Trachinotus ovatus and its application in toxicology and virus susceptibility.JOURNAL OF FISH BIOLOGY,90(5),1944-1959.
MLA Li, P,et al."Establishment and characterization of a cell line from the head kidney of golden pompano Trachinotus ovatus and its application in toxicology and virus susceptibility".JOURNAL OF FISH BIOLOGY 90.5(2017):1944-1959.

入库方式: OAI收割

来源:南海海洋研究所

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