Metabolic and fitness determinants for in vitro growth and intestinal colonization of the bacterial pathogen Campylobacter jejuni
文献类型：期刊论文
| 作者 | Gao, BL; Vorwerk, H; Huber, C; Lara-Tejero, M; Mohr, J; Goodman, AL; Eisenreich, W; Galan, JE; Hofreuter, D |
| 刊名 | PLOS BIOLOGY
 |
| 出版日期 | 2017 |
| 卷号 | 15期号:5页码:e2001390- |
| 通讯作者 | jorge.galan@yale.edu ; dirk.hofreuter@gmail.com |
| 中文摘要 | Campylobacter jejuni is one of the leading infectious causes of food-borne illness around the world. Its ability to persistently colonize the intestinal tract of a broad range of hosts, including food-producing animals, is central to its epidemiology since most infections are due to the consumption of contaminated food products. Using a highly saturated transposon insertion library combined with next-generation sequencing and a mouse model of infection, we have carried out a comprehensive genome-wide analysis of the fitness determinants for growth in vitro and in vivo of a highly pathogenic strain of C. jejuni. A comparison of the C. jejuni requirements to colonize the mouse intestine with those necessary to grow in different culture media in vitro, combined with isotopologue profiling and metabolic flow analysis, allowed us to identify its metabolic requirements to establish infection, including the ability to acquire certain nutrients, metabolize specific substrates, or maintain intracellular ion homeostasis. This comprehensive analysis has identified metabolic pathways that could provide the basis for the development of novel strategies to prevent C. jejuni colonization of food-producing animals or to treat human infections. |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/16281]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 推荐引用方式 GB/T 7714 | Gao, BL,Vorwerk, H,Huber, C,et al. Metabolic and fitness determinants for in vitro growth and intestinal colonization of the bacterial pathogen Campylobacter jejuni[J]. PLOS BIOLOGY,2017,15(5):e2001390-. |
| APA | Gao, BL.,Vorwerk, H.,Huber, C.,Lara-Tejero, M.,Mohr, J.,...&Hofreuter, D.(2017).Metabolic and fitness determinants for in vitro growth and intestinal colonization of the bacterial pathogen Campylobacter jejuni.PLOS BIOLOGY,15(5),e2001390-. |
| MLA | Gao, BL,et al."Metabolic and fitness determinants for in vitro growth and intestinal colonization of the bacterial pathogen Campylobacter jejuni".PLOS BIOLOGY 15.5(2017):e2001390-. |
入库方式： OAI收割
来源：南海海洋研究所
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