Identification of nocamycin biosynthetic gene cluster from Saccharothrix syringae NRRL B-16468 and generation of new nocamycin derivatives by manipulating gene cluster
文献类型：期刊论文
| 作者 | Mo, XH; Shi, CR; Gui, C; Zhang, YJ; Ju, JH; Wang, QJ |
| 刊名 | MICROBIAL CELL FACTORIES
 |
| 出版日期 | 2017 |
| 卷号 | 16页码:100- |
| 关键词 | Nocamycins Biosynthetic gene cluster Cytochrome P450 oxidase Post-tailoring modification Saccharothrix syringae |
| 通讯作者 | xhmo2013@163.com ; qingjiwang2016@hotmail.com |
| 中文摘要 | Background: Nocamycins I and II, produced by the rare actinomycete Saccharothrix syringae, belong to the tetramic acid family natural products. Nocamycins show potent antimicrobial activity and they hold great potential for antibacterial agent design. However, up to now, little is known about the exact biosynthetic mechanism of nocamycin. Results: In this report, we identified the gene cluster responsible for nocamycin biosynthesis from S. syringae and generated new nocamycin derivatives by manipulating its gene cluster. The biosynthetic gene cluster for nocamycin contains a 61 kb DNA locus, consisting of 21 open reading frames (ORFs). Five type I polyketide synthases (NcmAI, NcmAII, NcmAIII, NcmAIV, NcmAV) and a non-ribosomal peptide synthetase (NcmB) are proposed to be involved in synthesis of the backbone structure, a Dieckmann cyclase NcmC catalyze the releasing of linear chain and the formation of tetramic acid moiety, five enzymes (NcmEDGOP) are related to post-tailoring steps, and five enzymes (NcmNJKIM) function as regulators. Targeted inactivation of ncmB led to nocamycin production being completely abolished, which demonstrates that this gene cluster is involved in nocamycin biosynthesis. To generate new nocamycin derivatives, the gene ncmG, encoding for a cytochrome P450 oxidase, was inactivated. Two new nocamycin derivatives nocamycin III and nocamycin IV were isolated from the ncmG deletion mutant strain and their structures were elucidated by spectroscopic data analyses. Based on bioinformatics analysis and new derivatives isolated from gene inactivation mutant strains, a biosynthetic pathway of nocamycins was proposed. Conclusion: These findings provide the basis for further understanding of nocamycin biosynthetic mechanism, and set the stage to rationally engineer new nocamycin derivatives via combinatorial biosynthesis strategy. |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/16298]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 推荐引用方式 GB/T 7714 | Mo, XH,Shi, CR,Gui, C,et al. Identification of nocamycin biosynthetic gene cluster from Saccharothrix syringae NRRL B-16468 and generation of new nocamycin derivatives by manipulating gene cluster[J]. MICROBIAL CELL FACTORIES,2017,16:100-. |
| APA | Mo, XH,Shi, CR,Gui, C,Zhang, YJ,Ju, JH,&Wang, QJ.(2017).Identification of nocamycin biosynthetic gene cluster from Saccharothrix syringae NRRL B-16468 and generation of new nocamycin derivatives by manipulating gene cluster.MICROBIAL CELL FACTORIES,16,100-. |
| MLA | Mo, XH,et al."Identification of nocamycin biosynthetic gene cluster from Saccharothrix syringae NRRL B-16468 and generation of new nocamycin derivatives by manipulating gene cluster".MICROBIAL CELL FACTORIES 16(2017):100-. |
入库方式： OAI收割
来源：南海海洋研究所
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