Three rDNA Loci-Based Phylogenies of Tintinnid Ciliates (Ciliophora, Spirotrichea, Choreotrichida)
文献类型：期刊论文
| 作者 | Zhang, Qianqian1 ; Agatha, Sabine2; Zhang, Wuchang3; Dong, Jun1; Yu, Ying3; Jiao, Nianzhi4; Gong, Jun1
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| 刊名 | JOURNAL OF EUKARYOTIC MICROBIOLOGY
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| 出版日期 | 2017-03-01 |
| 卷号 | 64期号:2页码:226-241 |
| 关键词 | Barcoding genetic diversity marine protists microzooplankton multiple-locus phylogeny |
| ISSN号 | 1066-5234 |
| 产权排序 | [Zhang, Qianqian; Dong, Jun; Gong, Jun] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Agatha, Sabine] Salzburg Univ, Dept Ecol & Evolut, A-5020 Salzburg, Austria; [Zhang, Wuchang; Yu, Ying] Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China; [Jiao, Nianzhi] Xiamen Univ, State Key Lab Marine Environm Sci, Xiamen 361005, Peoples R China |
| 通讯作者 | Gong, J (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China. jgong@yic.ac.cn |
| 英文摘要 | To improve understanding of diversity, phylogeny and evolution in tintinnid ciliates, it is essential to link multiple molecular markers with properly identified and documented morphospecies. Accordingly, 54 tintinnid morphospecies/isolates mainly from the Yellow and East China Seas were collected and analysed. Using single-cell approaches, sequences were obtained for three rDNA loci (18S, ITS1-5.8S-ITS2, D1-D5 region of 28S). Twenty-six tintinnid morphospecies (29 isolates) are documented by micrographs, measurements, morphologically described, and compared with the original species description. Three rDNA loci-based phylogenetic analyses were then performed for these identified isolates. Sequences from 25 unidentified species/isolates were also included in the comparison of the three rDNA loci. Ribosomal DNA genes of the genus Leprotintinnus were analysed for the first time, showing that Leprotintinnus was closely related to Tintinnopsis radix and branched distinctly apart from the family Tintinnidiidae. Four novel clades (VI to IX) of the Tintinnopsis complex emerged in the 18S genealogies. Analyses of the relative variability in the ITS and 28S regions vs. the 18S rDNA showed that the ITS1-5.8S-ITS2 and ITS2 regions well co-varied with the 18S rDNA when the variations of the latter were less than 3%, whereas at difference of less than 1%, no correlation was found between the compared loci. These findings highlight the difficulties in using variable locus-based cut-off divergences in circumscribing tintinnid morphospecies. |
| 学科主题 | Microbiology |
| 研究领域[WOS] | Microbiology |
| 关键词[WOS] | RIBOSOMAL-RNA GENES ; SEQUENCE ALIGNMENT ; COASTAL WATERS ; OLIGOTRICH ; INSIGHTS ; MORPHOLOGY ; DIVERSITY ; EVOLUTION ; DNA ; HELICOSTOMELLA |
| 收录类别 | SCI |
| 原文出处 | 10.1111/jeu.12354 |
| 语种 | 英语 |
| WOS记录号 | WOS:000395079400009 |
| 源URL | [http://ir.yic.ac.cn/handle/133337/22018]  |
| 专题 | 烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 2.Salzburg Univ, Dept Ecol & Evolut, A-5020 Salzburg, Austria 3.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 4.Xiamen Univ, State Key Lab Marine Environm Sci, Xiamen 361005, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Qianqian,Agatha, Sabine,Zhang, Wuchang,et al. Three rDNA Loci-Based Phylogenies of Tintinnid Ciliates (Ciliophora, Spirotrichea, Choreotrichida)[J]. JOURNAL OF EUKARYOTIC MICROBIOLOGY,2017,64(2):226-241. |
| APA | Zhang, Qianqian.,Agatha, Sabine.,Zhang, Wuchang.,Dong, Jun.,Yu, Ying.,...&Gong, Jun.(2017).Three rDNA Loci-Based Phylogenies of Tintinnid Ciliates (Ciliophora, Spirotrichea, Choreotrichida).JOURNAL OF EUKARYOTIC MICROBIOLOGY,64(2),226-241. |
| MLA | Zhang, Qianqian,et al."Three rDNA Loci-Based Phylogenies of Tintinnid Ciliates (Ciliophora, Spirotrichea, Choreotrichida)".JOURNAL OF EUKARYOTIC MICROBIOLOGY 64.2(2017):226-241. |
入库方式： OAI收割
来源：烟台海岸带研究所
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