Characterization of Lipopeptide Biosurfactants Produced by Bacillus licheniformis MB01 from Marine Sediments
文献类型：期刊论文
| 作者 | Chen, Yulin1,2; Liu, Shiliang A.3; Mou, Haijin2; Ma, Yunxiao2; Li, Meng1; Hu, Xiaoke1
|
| 刊名 | FRONTIERS IN MICROBIOLOGY
 |
| 出版日期 | 2017-05-16 |
| 卷号 | 8 |
| 关键词 | Bacillus licheniformis purification bacteriostat structure LC-ESI-MS/MS stabilization |
| ISSN号 | 1664-302X |
| 英文摘要 | Antibiotic resistance has become one of the world's most severe problems because of the overuse of antibiotics. Antibiotic-resistant bacteria are more difficult to kill and more expensive to treat. Researchers have been studied on antibiotic alternatives such as antimicrobial peptides and lipopeptides. A functional bacteria MB01 producing lipopeptides which can be used as bacteriostat was isolated from the Bohai Sea sediments, which had been identified as Bacillus licheniformis by the morphological, physiological, and biochemical identification and 16s rDNA sequence. The lipopeptides produced by MB01 were determined to be cyclic surfactin homologs by LC-ESI-MS structural identification after crude extraction and LH-20 chromatography. [M + H](+) m/z 994, 1008, 1022, and 1036 were all the characteristic molecular weight of surfactin homologs. CID analysis revealed that the molecular structure of the lipopeptides was R-n-Glu(1)-Leu/lle(2)-Leu(3)-Val(4)-Asp(5)-Leu(6)-Leu/Ile(7). The lipopeptides showed well resistance to UV light and the change of pH and temperature. |
| 研究领域[WOS] | Microbiology |
| 关键词[WOS] | ANTIMICROBIAL PEPTIDES ; CYCLIC LIPOPEPTIDES ; VIBRIO-PARAHAEMOLYTICUS ; MS/MS CHARACTERIZATION ; MICROBIAL SURFACTANTS ; MASS-SPECTROMETRY ; IDENTIFICATION ; PURIFICATION ; SUBTILIS ; STRAINS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000401575500003 |
| 源URL | [http://ir.yic.ac.cn/handle/133337/22514]  |
| 专题 | 烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai, Peoples R China 2.Ocean Univ China, Sch Food Sci & Engn, Qingdao, Peoples R China 3.Louisiana State Univ, Sch Vet Med, Baton Rouge, LA 70803 USA |
| 推荐引用方式 GB/T 7714 | Chen, Yulin,Liu, Shiliang A.,Mou, Haijin,et al. Characterization of Lipopeptide Biosurfactants Produced by Bacillus licheniformis MB01 from Marine Sediments[J]. FRONTIERS IN MICROBIOLOGY,2017,8. |
| APA | Chen, Yulin,Liu, Shiliang A.,Mou, Haijin,Ma, Yunxiao,Li, Meng,&Hu, Xiaoke.(2017).Characterization of Lipopeptide Biosurfactants Produced by Bacillus licheniformis MB01 from Marine Sediments.FRONTIERS IN MICROBIOLOGY,8. |
| MLA | Chen, Yulin,et al."Characterization of Lipopeptide Biosurfactants Produced by Bacillus licheniformis MB01 from Marine Sediments".FRONTIERS IN MICROBIOLOGY 8(2017). |
入库方式： OAI收割
来源：烟台海岸带研究所
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