Comparative analysis of microbial communities during enrichment and isolation of DDT-degrading bacteria by culture-dependent and -independent methods
文献类型：期刊论文
| 作者 | Wang, Beibei1,2; Liu, Wuxing1; Liu, Xiaoyan1,2; Franks, Ashley E.3; Teng, Ying1; Luo, Yongming1,4
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| 刊名 | SCIENCE OF THE TOTAL ENVIRONMENT
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| 出版日期 | 2017-07-15 |
| 卷号 | 590页码:297-303 |
| 关键词 | DDT-degrading microorganisms Microbial community Culture-dependent Culture-independent Community diversity |
| ISSN号 | 0048-9697 |
| 产权排序 | [Wang, Beibei; Liu, Wuxing; Liu, Xiaoyan; Teng, Ying; Luo, Yongming] Chinese Acad Sci, Inst Soil Sci, Key Lab Soil Environm & Pollut Remediat, Nanjing 210008, Jiangsu, Peoples R China; [Wang, Beibei; Liu, Xiaoyan] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Franks, Ashley E.] La Trobe Univ, Dept Physiol Anat & Microbiol, Melbourne, Vic 3086, Australia; [Luo, Yongming] Chinese Acad Sci, Inst Coastal Zone Res, Yantai 264003, Peoples R China |
| 通讯作者 | Liu, WX (reprint author), Chinese Acad Sci, Inst Soil Sci, Key Lab Soil Environm & Pollut Remediat, Nanjing 210008, Jiangsu, Peoples R China. liuwuxin@issas.ac.cn |
| 英文摘要 | Microcosms for enrichment of DDT degrading microorganisms were monitored using culture-dependent and -independent methods. Culture dependent methods isolated several strains with DDT degradation potential, Pseudomonas species being the most frequent. One isolate, Streptomyces sp. strain D3, had a degradation rate of 77% with 20 mg L-1 of DDT after 7 days incubation, D3 also had degradation rates of 75% and 30% for PCB77 (3,3',4,4'-tetrachloro biphenyl) and PCNB (pentachloronitrobenzene) respectively. Culture-independent high throughput sequencing identified a different subset of the microbial community within the enrichment microcosms to the culture dependent method. Pseudomonas, the most frequently isolated strain, only represented the 12th most abundant operational taxonomic unit in the sequencing dataset (relative abundance 0.9%). The most frequently observed bacterial genus in the culture-independent analysis did not correspond with those recovered by culture-dependent methods. These results suggested that deep sequencing followed by a targeted isolation approach might provide an advantageous route to bioremediation studies. (C) 2017 Elsevier B.V. All rights reserved. |
| 学科主题 | Environmental Sciences & Ecology |
| 研究领域[WOS] | Environmental Sciences & Ecology |
| 关键词[WOS] | ORGANOCHLORINE PESTICIDES ; SEA BACTERIOPLANKTON ; AGRICULTURAL SOILS ; CONTAMINATED SOIL ; DEGRADATION ; CHINA ; WATER ; BIODEGRADATION ; DIVERSITY ; PENTACHLORONITROBENZENE |
| 收录类别 | SCI |
| 原文出处 | 10.1016/j.scitotenv.2017.03.004 |
| 语种 | 英语 |
| WOS记录号 | WOS:000399511800031 |
| 源URL | [http://ir.yic.ac.cn/handle/133337/22047]  |
| 专题 | 烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Soil Sci, Key Lab Soil Environm & Pollut Remediat, Nanjing 210008, Jiangsu, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.La Trobe Univ, Dept Physiol Anat & Microbiol, Melbourne, Vic 3086, Australia 4.Chinese Acad Sci, Inst Coastal Zone Res, Yantai 264003, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Beibei,Liu, Wuxing,Liu, Xiaoyan,et al. Comparative analysis of microbial communities during enrichment and isolation of DDT-degrading bacteria by culture-dependent and -independent methods[J]. SCIENCE OF THE TOTAL ENVIRONMENT,2017,590:297-303. |
| APA | Wang, Beibei,Liu, Wuxing,Liu, Xiaoyan,Franks, Ashley E.,Teng, Ying,&Luo, Yongming.(2017).Comparative analysis of microbial communities during enrichment and isolation of DDT-degrading bacteria by culture-dependent and -independent methods.SCIENCE OF THE TOTAL ENVIRONMENT,590,297-303. |
| MLA | Wang, Beibei,et al."Comparative analysis of microbial communities during enrichment and isolation of DDT-degrading bacteria by culture-dependent and -independent methods".SCIENCE OF THE TOTAL ENVIRONMENT 590(2017):297-303. |
入库方式： OAI收割
来源：烟台海岸带研究所
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