中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
样品前处理方式对沉积物酶水解有机磷的影响

文献类型:期刊论文

作者张友; 吕迎春; 高丽; 刘书花; 徐刚; 王安东; 赵亚杰
刊名环境化学
出版日期2017-02-15
卷号36期号:1页码:107-113
关键词沉积物 磷酸酶 有机磷 提取条件
ISSN号0254-6108
其他题名Effect of sample pretreatment on enzymatic hydrolysis of organic phosphorus in sediment
产权排序(1)中国科学院烟台海岸带研究所;(2)中国科学院大学;(3)烟台大学;(4)山东省分析测试中心;(5)山东省黄河三角洲国家级自然保护区管理局;
通讯作者徐刚
中文摘要磷酸酶水解技术是研究沉积物有机磷形态和生物有效性的有力工具.沉积物样品处理过程对酶水解有机磷的影响尚不清楚.为此,以天鹅湖表层沉积物为研究对象,研究了干燥方式、提取剂、提取比例、提取时间条件对沉积物酶水解有机磷的影响特征,以寻求最佳的酶水解技术的实验条件.研究结果表明,自然风干处理引发了有机磷水解,降低了磷酸二酯磷(Diester-P)含量.而冻干处理更真实地保存了沉积物有机磷形态特征,比较合适有机磷分析.尽管HCl预提取显著增大了有机磷的提取比例,但是存在Diester-P水解风险,因此Na OH/EDTA一步提取更适合有机磷的测定.较长的提取时间和较大固液比同样也显著增大了有机磷的提取效率,但是同样存在Diester-P水解风险.研究显示Diester-P极不稳定,易受实验条件的影响,在实际操作中需要特别注意.总之沉积物样品采用冷冻干燥处理,固液比1∶30,采用0.25 mol·L-1Na OH+50 mmol·L-1EDTA提8 h更适合沉积物酶水解有机磷的分析. 
英文摘要Enzymatic hydrolysis is a powerful tool to study the speciation and bioavailability ofsediment organic phosphorus ( P) £® However£¬the effects of the sample treatment on the enzymatichydrolysis need to be fully understood£® In order to investigate the optimal condition for enzymatichydrolysis£¬the surface sediments of Swan Lake were collected to study the effects of samplepreparation procedures£¬including sample pretreatment£¬extraction solution£¬extraction time£¬andratio of sample to extraction efficiency on enzymatic hydrolysis£® The results showed that air-dryingcaused the hydrolysis of diester-P in sediments£® Freeze drying preserved the characteristics of organicphosphorus£¬and is recommended for the study of organic phosphorus in sediments£® Although the recovery of P was significantly increased by HCl pre-extraction,there is also a risk of diester-Phydrolysis at the same time. As a result,one step extraction of NaOH/EDTA is recommended forenzymatic hydrolysis of organic phosphorus in sediment. In addition,longer extraction time andhigher extraction ratio ( S ( sediment ) /V ( volume ) ) tend to increased the risk of diester-Phydrolysis although they increased the recovery of P from sediment. Our results suggest that diester-Pin sediment is extremely unstable and vulnerable to extraction conditions. Thus it deserves specialcaution with sample analysis and data interpretation. In conclusion,the freeze dried sediments wereextracted with 0.25 mol·L-1 NaOH+50 mmol·L-1 EDTA at 8 h and sediment /solution ratio of 1∶30which were recommended for enzymatic hydrolysis of organic phosphorus in sediments.
收录类别CSCD
语种中文
CSCD记录号CSCD:5946176
源URL[http://ir.yic.ac.cn/handle/133337/22081]  
专题烟台海岸带研究所_污染过程与控制实验室
烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
作者单位1.中国科学院烟台海岸带研究所
2.中国科学院大学
3.烟台大学
4.山东省分析测试中心
5.山东省黄河三角洲国家级自然保护区管理局
推荐引用方式
GB/T 7714
张友,吕迎春,高丽,等. 样品前处理方式对沉积物酶水解有机磷的影响[J]. 环境化学,2017,36(1):107-113.
APA 张友.,吕迎春.,高丽.,刘书花.,徐刚.,...&赵亚杰.(2017).样品前处理方式对沉积物酶水解有机磷的影响.环境化学,36(1),107-113.
MLA 张友,et al."样品前处理方式对沉积物酶水解有机磷的影响".环境化学 36.1(2017):107-113.

入库方式: OAI收割

来源:烟台海岸带研究所

浏览0
下载0
收藏0
其他版本

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。