Understanding regulation of microRNAs on intestine regeneration in the sea cucumber Apostichopus japonicus using high-throughput sequencing
文献类型：期刊论文
| 作者 | Sun, Lina1; Sun, Jingchun1; Li, Xiaoni1,2; Zhang, Libin1; Yang, Hongsheng2; Wang, Qing3
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| 刊名 | COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS
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| 出版日期 | 2017-06-01 |
| 卷号 | 22页码:1-9 |
| ISSN号 | 1744-117X |
| 关键词 | Sea cucumber MicroRNAs miRNA expression profiles Intestine regeneration |
| 英文摘要 | The sea cucumber, as a member of the Echinodermata, has the capacity to restore damaged organs and body parts, which has always been a key scientific issue. MicroRNAs (miRNAs), a class of short noncoding RNAs, play important roles in regulating gene expression. In the present study, we applied high-throughput sequencing to investigate alterations of miRNA expression in regenerative intestine compared to normal intestine. A total of 73 differentially expressed miRNAs were obtained, including 59 up-regulated miRNAs and 14 down-regulated miRNAs. Among these molecules, Aja-miR-1715-5p, Aja-miR-153, Aja-miR-252a, Aja-miR-153-5p, Aja-miR-252b, Aja-miR-2001, Aja-miR-64d-3p, and Aja-miR-252-5p were differentially expressed over 10-fold at 3 days post-evisceration (dpe). Notably, real-time PCR revealed that Aja-miR-1715-5p was up-regulated 1390 fold at 3 dpe. Moreover, putative target gene co-expression analyses, gene ontology, and pathway analyses suggest that these miRNAs play important roles in specific cellular events (cell proliferation, migration, and apoptosis), metabolic regulation, and energy redistribution. These results will provide a basis for future studies of miRNA regulation in sea cucumber regeneration. (C) 2017 Elsevier Inc. All rights reserved. |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Genetics & Heredity |
| 关键词[WOS] | HOLOTHURIA-GLABERRIMA ; CELL-PROLIFERATION ; DOWN-REGULATION ; VISCERAL REGENERATION ; EXPRESSION ; GENE ; CANCER ; DROSOPHILA ; MIR-133 ; DEDIFFERENTIATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000402220500001 |
| 源URL | [http://ir.yic.ac.cn/handle/133337/22511]  |
| 专题 | 烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remedia, Yantai, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, Lina,Sun, Jingchun,Li, Xiaoni,et al. Understanding regulation of microRNAs on intestine regeneration in the sea cucumber Apostichopus japonicus using high-throughput sequencing[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS,2017,22:1-9. |
| APA | Sun, Lina,Sun, Jingchun,Li, Xiaoni,Zhang, Libin,Yang, Hongsheng,&Wang, Qing.(2017).Understanding regulation of microRNAs on intestine regeneration in the sea cucumber Apostichopus japonicus using high-throughput sequencing.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS,22,1-9. |
| MLA | Sun, Lina,et al."Understanding regulation of microRNAs on intestine regeneration in the sea cucumber Apostichopus japonicus using high-throughput sequencing".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS 22(2017):1-9. |
入库方式： OAI收割
来源：烟台海岸带研究所
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