Molecular characterization, expression and antimicrobial activities of two c-type lysozymes from manila clam Venerupis philippinarum
文献类型：期刊论文
| 作者 | Yang, Dinglong1,2,3 ; Wang, Qing1; Cao, Ruiwen1,3; Chen, Lizhu1,3; Liu, Yongliang2; Cong, Ming1; Wu, Huifeng1; Li, Fei1; Ji, Chenglong1; Zhao, Jianmin1,2
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| 刊名 | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
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| 出版日期 | 2017-08-01 |
| 卷号 | 73页码:109-118 |
| 关键词 | Venerupis philippinarum C-type lysozyme Antimicrobial activity Muramidase activity |
| ISSN号 | 0145-305X |
| 英文摘要 | Lysozymes play an important role in the innate immune responses with which mollusks respond to bacterial invasion through its lytic activity. In the present study, two c-type lysozymes (designed as VpCLYZ-1 and VpCLYZ-2, respectively) were identified and characterized from the manila clam Venerupis philippinarum. The full-length cDNA of VpCLYZ-1 and VpCLYZ-2 was of 629 and 736 bp, encoding a polypeptide of 156 and153 amino acid residues, respectively. The deduced amino acid sequences of VpCLYZs showed high similarity to other known invertebrate c-type lysozymes. Multiple alignments and phylogenetic relationship strongly suggested that VpCLYZ-1 and VpCLYZ-2 belonged to the c-type lysozyme family. Both VpCLYZ-1 and VpCLYZ-2 transcripts were constitutively expressed in a wide range of tissues with different levels. The VpCLYZ-1 transcript was dominantly expressed in hepatopancreas and hemocytes, while VpCLYZ-2 transcript was mainly expressed in the tissues of hepatopancreas and gills. Both the mRNA expression of VpCLYZ-1 and VpCLYZ-2 was significantly up-regulated at 12 h post Vibrio anguillarum challenge. The recombinant VpCLYZ-1 and VpCLYZ-2 (designed as rVpCLYZ-1 and rVpCLYZ-2) exhibited lytic activity against all tested bacteria, and rVpCLYZ-1 showed higher activities than rVpCLYZ-2 in killing Micrococcus luteus and V anguillarum. Overall, our results suggested that VpCLYZ-1 and VpCLYZ-2 belonged to the c-type lysozyme family, and played important roles in the immune responses of manila clam, especially in the elimination of pathogens. (C) 2017 Elsevier Ltd. All rights reserved. |
| 研究领域[WOS] | Immunology ; Zoology |
| 关键词[WOS] | BROWN RING DISEASE ; RUDITAPES-PHILIPPINARUM ; ANTIBACTERIAL ACTIVITY ; EASTERN OYSTER ; CLONING ; IDENTIFICATION ; PURIFICATION ; HEMOCYTES ; SYSTEM ; ANDREI |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000401380400013 |
| 源URL | [http://ir.yic.ac.cn/handle/133337/22512]  |
| 专题 | 烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室 海岸带生物资源高效利用研究与发展中心 |
| 作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Zone Environm Proc, Yantai 264003, Shandong, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Muping Coastal Environm Res Stn, Yantai 264003, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Dinglong,Wang, Qing,Cao, Ruiwen,et al. Molecular characterization, expression and antimicrobial activities of two c-type lysozymes from manila clam Venerupis philippinarum[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2017,73:109-118. |
| APA | Yang, Dinglong.,Wang, Qing.,Cao, Ruiwen.,Chen, Lizhu.,Liu, Yongliang.,...&Zhao, Jianmin.(2017).Molecular characterization, expression and antimicrobial activities of two c-type lysozymes from manila clam Venerupis philippinarum.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,73,109-118. |
| MLA | Yang, Dinglong,et al."Molecular characterization, expression and antimicrobial activities of two c-type lysozymes from manila clam Venerupis philippinarum".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 73(2017):109-118. |
入库方式： OAI收割
来源：烟台海岸带研究所
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