Lysine acetylation regulates the activity of Escherichia coli pyridoxine 5 '-phosphate oxidase
文献类型：期刊论文
| 作者 | Gu, Jing1; Chen, Yuanyuan1,2; Guo, Hongsen1,2; Sun, Manluan1,3; Yang, Mingkun4; Wang, Xude1; Zhang, Xian'en5; Deng, Jiaoyu1 |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2017-02-01 |
| 卷号 | 49期号:2页码:186-192 |
| 关键词 | pyridoxine 5 '-phosphate oxidase lysine acetylation CobB deacetylation |
| ISSN号 | 1672-9145 |
| 英文摘要 | N epsilon-lysine acetylation is one of the most abundant post-translational modifications in eukaryote and prokaryote. Protein acetylome of Escherichia coli has been screened using mass spectrometry (MS) technology, and many acetylated proteins have been identified, including the pyridoxine 5'-phosphate oxidase (EcPNPOx), but the biological roles played by lysine acetylation in EcPNPOx still remain unknown. In this study, EcPNPOx was firstly overexpressed and purified, and two acetylated lysine residues were identified by the subsequent liquid chromatography-tandem mass spectrometry analysis. Site-directed mutagenesis analysis demonstrated that acetylated lysine residues play important roles in the enzymatic activity and enzymatic properties of the protein. EcPNPOx could be non-enzymatically acetylated by acetyl-phosphate and deacetylated by CobB in vitro. Furthermore, enzymatic activities of acetylated and deacetylated EcPNPOx were compared in vitro, and results showed that acetylation led to a decrease of its enzymatic activity, which could be rescued by CobB deacetylation. Taken together, our data suggest that CobB modulates the enzymatic activity of EcPNPOx in vitro. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | X-RAY-STRUCTURE ; PROTEIN ACETYLATION ; PYRIDOXINE-5'-PHOSPHATE OXIDASE ; 5-PHOSPHATE OXIDASE ; ANGSTROM RESOLUTION ; RABBIT LIVER ; E. COLI ; COBB ; BIOSYNTHESIS ; VITAMIN-B6 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000397088800010 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/29053]  |
| 专题 | 水生生物研究所_水环境工程研究中心_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, Wuhan 430071, Peoples R China 2.Huazhong Agr Univ, Wuhan 430070, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100039, Peoples R China 4.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430071, Peoples R China 5.Chinese Acad Sci, Nat Lab Biomacromolecules, Inst Biophys, Beijing 100101, Peoples R China |
| 推荐引用方式 GB/T 7714 | Gu, Jing,Chen, Yuanyuan,Guo, Hongsen,et al. Lysine acetylation regulates the activity of Escherichia coli pyridoxine 5 '-phosphate oxidase[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2017,49(2):186-192. |
| APA | Gu, Jing.,Chen, Yuanyuan.,Guo, Hongsen.,Sun, Manluan.,Yang, Mingkun.,...&Deng, Jiaoyu.(2017).Lysine acetylation regulates the activity of Escherichia coli pyridoxine 5 '-phosphate oxidase.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,49(2),186-192. |
| MLA | Gu, Jing,et al."Lysine acetylation regulates the activity of Escherichia coli pyridoxine 5 '-phosphate oxidase".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 49.2(2017):186-192. |
入库方式： OAI收割
来源：水生生物研究所
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