Zebrafish akt2 is essential for survival, growth, bone development, and glucose homeostasis
文献类型：期刊论文
| 作者 | Zhang, Dawei1; Wang, Jing1; Zhou, Chi1; Xiao, Wuhan1,2,3 |
| 刊名 | MECHANISMS OF DEVELOPMENT
 |
| 出版日期 | 2017-02-01 |
| 卷号 | 143页码:42-52 |
| 关键词 | Akt2 Zebrafish Glucose homeostasis Fin ray development |
| ISSN号 | 0925-4773 |
| 英文摘要 | As one of three akt isoforms, akt2 plays a key role in the regulation of widely divergent cellular processes in mammals. However, its role and underlying mechanisms in zebrafish remain largely unknown. To elucidate the function of akt2 in zebrafish, we generated zebrafish lacking akt2 gene via CRISPR/Cas9 technology. Akt2-null zebrafish exhibit partial lethality and severe growth deficiency, which is different from those observed in akt2-null mice. Furthermore, akt2-null zebrafish display deficiency in fin ray development, but their cartilage is not affected. Similar to observations in akt2-nullmice, akt2-null zebrafish display impaired glucose homeostasis. However, in contrast to that in akt2-nullmice, insulin level is lower in akt2-null zebrafish, implicating the symptoms of type I diabetes exhibited in akt2-null zebrafish. In addition, transcriptome analysis reveals that the genes involved in metabolism and osteogenesis are disturbed in akt2-null zebrafish. Taken together, these data not only support an important role of akt2 in zebrafish survival, growth, bone development and glucose homeostasis, but also suggest that akt2 has divergent functions between mice and zebrafish, even though they are evolutionarily conserved. (C) 2017 Elsevier Ireland Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Developmental Biology |
| 研究领域[WOS] | Developmental Biology |
| 关键词[WOS] | AKT/PROTEIN KINASE-B ; MICE LACKING ; SKELETAL-MUSCLE ; OSTEOBLAST DIFFERENTIATION ; INSULIN-RESISTANCE ; IN-VITRO ; GAMMA ; GENE ; RNA ; DEFICIENCY |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000395505200006 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/29044]  |
| 专题 | 水生生物研究所_水生生物分子与细胞生物学研究中心_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 3.Minist Agr, Key Lab Aquaculture Dis Control, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Dawei,Wang, Jing,Zhou, Chi,et al. Zebrafish akt2 is essential for survival, growth, bone development, and glucose homeostasis[J]. MECHANISMS OF DEVELOPMENT,2017,143:42-52. |
| APA | Zhang, Dawei,Wang, Jing,Zhou, Chi,&Xiao, Wuhan.(2017).Zebrafish akt2 is essential for survival, growth, bone development, and glucose homeostasis.MECHANISMS OF DEVELOPMENT,143,42-52. |
| MLA | Zhang, Dawei,et al."Zebrafish akt2 is essential for survival, growth, bone development, and glucose homeostasis".MECHANISMS OF DEVELOPMENT 143(2017):42-52. |
入库方式： OAI收割
来源：水生生物研究所
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