Building a multipurpose insertional mutant library for forward and reverse genetics in Chlamydomonas
文献类型：期刊论文
| 作者 | Cheng, Xi1; Liu, Gai1; Ke, Wenting1; Zhao, Lijuan1,2; Lv, Bo1,2; Ma, Xiaocui1,2; Xu, Nannan1,2; Xia, Xiaoling1; Deng, Xuan1; Zheng, Chunlei3 |
| 刊名 | PLANT METHODS
 |
| 出版日期 | 2017-05-15 |
| 卷号 | 13 |
| 关键词 | Chlamydomonas Insertional mutants Mutant library Flagella Intraflagellar transport Oil droplet |
| ISSN号 | 1746-4811 |
| 英文摘要 | Background: The unicellular green alga, Chlamydomonas reinhardtii, is a classic model for studying flagella and biofuel. However, precise gene editing, such as Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR-associated protein (Cas9) system, is not widely used in this organism. Screening of random insertional mutant libraries by polymerase chain reaction provides an alternate strategy to obtain null mutants of individual gene. But building, screening, and maintaining such a library was time-consuming and expensive. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemical Research Methods ; Plant Sciences |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Plant Sciences |
| 关键词[WOS] | NUCLEAR TRANSFORMATION ; SELECTABLE MARKER ; GREEN-ALGA ; REINHARDTII ; EXPRESSION ; MODEL ; RESISTANCE ; PROTEINS ; DNA ; COMPLEMENTATION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000401181400001 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/29186]  |
| 专题 | 水生生物研究所_其他_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan 430072, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China 3.Hubei Univ, Coll Life Sci, Wuhan 430062, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cheng, Xi,Liu, Gai,Ke, Wenting,et al. Building a multipurpose insertional mutant library for forward and reverse genetics in Chlamydomonas[J]. PLANT METHODS,2017,13. |
| APA | Cheng, Xi.,Liu, Gai.,Ke, Wenting.,Zhao, Lijuan.,Lv, Bo.,...&Huang, Kaiyao.(2017).Building a multipurpose insertional mutant library for forward and reverse genetics in Chlamydomonas.PLANT METHODS,13. |
| MLA | Cheng, Xi,et al."Building a multipurpose insertional mutant library for forward and reverse genetics in Chlamydomonas".PLANT METHODS 13(2017). |
入库方式： OAI收割
来源：水生生物研究所
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