iTRAQ reveals proteomic changes during intestine regeneration in the sea cucumber Apostichopus japonicus
文献类型：期刊论文
| 作者 | Sun, Lina1; Xu, Dongxue3 ; Xu, Qinzeng4; Sun, Jingchun1; Xing, Lili1,2; Zhang, Libin1; Yang, Hongsheng1
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| 刊名 | COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS
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| 出版日期 | 2017-06-01 |
| 卷号 | 22页码:39-49 |
| 关键词 | Sea cucumber Intestine regeneration iTRAQ Proteomics |
| 英文摘要 | Sea cucumbers have a striking capacity to regenerate most of their viscera after evisceration, which has drawn the interest of many researchers. In this study, the isobaric tag for relative and absolute quantitation (iTRAQ) was utilized to investigate protein abundance changes during intestine regeneration in sea cucumbers. A total of 4073 proteins were identified, and 2321 proteins exhibited significantly differential expressions, with 1100 upregulated and 1221 downregulated proteins. Our results suggest that intestine regeneration constitutes a complex life activity regulated by the cooperation of various biological processes, including cytoskeletal changes, extracellular matrix (ECM) remodeling and ECM-receptor interactions, protein synthesis, signal recognition and transduction, energy production and conversion, and substance transport and metabolism. Additionally, real-time PCR showed mRNA expression of differentially expressed genes correlated positively with their protein levels. Our results provided a basis for studying the regulatory mechanisms associated with sea cucumber regeneration. (C) 2017 Elsevier Inc All rights reserved. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000402220500005 |
| 版本 | 出版稿 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/136873]  |
| 专题 | 海洋研究所_海洋生态与环境科学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Qingdao Agr Univ, Marine Sci & Engn Coll, Qingdao, Peoples R China 4.State Ocean Adm, Inst Oceanog 1, Key Lab Marine Ecol & Environm Sci & Engn, Qingdao, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, Lina,Xu, Dongxue,Xu, Qinzeng,et al. iTRAQ reveals proteomic changes during intestine regeneration in the sea cucumber Apostichopus japonicus[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS,2017,22:39-49. |
| APA | Sun, Lina.,Xu, Dongxue.,Xu, Qinzeng.,Sun, Jingchun.,Xing, Lili.,...&Yang, Hongsheng.(2017).iTRAQ reveals proteomic changes during intestine regeneration in the sea cucumber Apostichopus japonicus.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS,22,39-49. |
| MLA | Sun, Lina,et al."iTRAQ reveals proteomic changes during intestine regeneration in the sea cucumber Apostichopus japonicus".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS 22(2017):39-49. |
入库方式： OAI收割
来源：海洋研究所
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