中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Synchronous determination with double-wavelength by RP-HPLC-UV and optimization of ultrasound-assisted extraction of phenolic acids from Caragana species using response surface methodology

文献类型:期刊论文

作者Zeng, Zhi1,3; Ji, Zhongyin1,3; Hu, Na1; Chen, Shasha1,3; Bai, Bo1,2; Wang, Honglun1,2; Suo, Yourui1,2
刊名journal of pharmaceutical and biomedical analysis
出版日期2017-06-05
卷号140页码:182-189
关键词Response surface methodology (RSM) RP-HPLC-UV Phenolic acids Caragana korshinskii Kom
英文摘要the utilization of caragana korshinskii kom. (ck) is currently concentrated on its ecological and fuel functions. little attention has been devoted to the analysis of their phenolic acid (pa) components. to obtain more data for further utilization of ck, a new analysis protocol was tested to determine pas synchronously by rp-hplc-uv with double-wavelength (280 nm and 320 nm) detection. specifically, separation of pa components was performed on a hypersil gold c-18 reverse phase column with gradient elution. a four factor-three-level box-behnken design was implemented for optimization of pa extraction. the results demonstrated that ck were rich primarily in chlorogenic acid, vanillic acid, caffeic acid and rosmarinic acid. the total content of pas in ck leaves was the highest compared with its other parts. the distribution of total flavonoid content of ck was leaves > flowers > bark, while that of the total phenolic content of ck was flowers > leaves > bark. (c) 2017 elsevier b.v. all rights reserved.
WOS标题词science & technology ; physical sciences ; life sciences & biomedicine
类目[WOS]chemistry, analytical ; pharmacology & pharmacy
研究领域[WOS]chemistry ; pharmacology & pharmacy
关键词[WOS]performance liquid-chromatography ; antioxidant activity ; fluorescence detection ; fatty-acids ; olive oil ; constituents ; flavonoids ; berries ; apples ; leaves
收录类别SCI
语种英语
WOS记录号WOS:000402850500022
源URL[http://ir.nwipb.ac.cn/handle/363003/7014]  
专题西北高原生物研究所_中国科学院西北高原生物研究所
作者单位1.Chinese Acad Sci, Northwest Plateau Inst Biol, Key Lab Tibetan Med Res, Xining 810008, Peoples R China
2.Qinghai Univ, State Key Lab Plateau Ecol & Agr, Xining 810016, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714
Zeng, Zhi,Ji, Zhongyin,Hu, Na,et al. Synchronous determination with double-wavelength by RP-HPLC-UV and optimization of ultrasound-assisted extraction of phenolic acids from Caragana species using response surface methodology[J]. journal of pharmaceutical and biomedical analysis,2017,140:182-189.
APA Zeng, Zhi.,Ji, Zhongyin.,Hu, Na.,Chen, Shasha.,Bai, Bo.,...&Suo, Yourui.(2017).Synchronous determination with double-wavelength by RP-HPLC-UV and optimization of ultrasound-assisted extraction of phenolic acids from Caragana species using response surface methodology.journal of pharmaceutical and biomedical analysis,140,182-189.
MLA Zeng, Zhi,et al."Synchronous determination with double-wavelength by RP-HPLC-UV and optimization of ultrasound-assisted extraction of phenolic acids from Caragana species using response surface methodology".journal of pharmaceutical and biomedical analysis 140(2017):182-189.

入库方式: OAI收割

来源:西北高原生物研究所

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