The WRKY transcription factors PtrWRKY18 and PtrWRKY35 promote Melampsora resistance in Populus
文献类型：期刊论文
| 作者 | Jiang, Yuanzhong1,2; Guo, Li1; Ma, Xiaodong3,4; Zhao, Xin1; Jiao, Bo1; Li, Chaofeng1,3; Luo, Keming1 |
| 刊名 | tree physiology
 |
| 出版日期 | 2017-05-01 |
| 卷号 | 37期号:5页码:665-675 |
| 关键词 | CRISPER Cas9 fungal pathogen plant defense Populus WRKY transcription factor |
| 英文摘要 | wrky transcription factors play important roles in response to diverse environmental stresses, but exact functions of these proteins in poplar defense are still largely unknown. in a previous study, we have shown that poplar wrky89 is induced by salicylic acid (sa) treatment and plays an important role in resistance against fungi in transgenic poplars. here, we determined an increase in transcript levels of group iia wrky members in transgenic poplars overexpressing wrky89 using quantitative real-time polymerase chain reaction analysis. yeast one-hybrid assay showed that ptrwrky18 and ptrwrky35 were potential target genes of wrky89. furthermore, we demonstrated that ptrwrky18 and ptrwrky35 were localized in the nucleus, and exhibited no transcription activation activity. constitutive overexpression of ptrwrky18 and ptrwrky35 in poplars activated pathogenesis-related genes, and increased resistance to the biotrophic pathogen melampsora. the results also provided support for the involvement of sa-mediated signaling in melampsora resistance. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | forestry |
| 研究领域[WOS] | forestry |
| 关键词[WOS] | multiple sequence alignment ; plant-disease resistance ; salicylic-acid ; arabidopsis-thaliana ; active oxygen ; defense ; expression ; immunity ; tolerance ; synthase |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000402617800011 |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/7019]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Southwest Univ, Key Lab Ecoenvironm Three Gorges Reservoir Reg, Minist Educ,Inst Resources Bot,Sch Life Sci, Chongqing Key Lab Transgen Plant & Safety Control, 1 Tiansheng Rd, Chongqing 400715, Peoples R China 2.Sichuan Univ, MOE Key Lab Bioresources & Ecoenvironm, Coll Life Sci, 24,South Sect 1,Yihuan Rd, Chengdu 610065, Peoples R China 3.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, 23 Xinning Rd, Xining 810008, Qinghai, Peoples R China 4.Qinghai Univ Nationalities, Sch Chem & Chem Engn, 3 Bayi Mid Rd, Xining 810007, Qinghai, Peoples R China |
| 推荐引用方式 GB/T 7714 | Jiang, Yuanzhong,Guo, Li,Ma, Xiaodong,et al. The WRKY transcription factors PtrWRKY18 and PtrWRKY35 promote Melampsora resistance in Populus[J]. tree physiology,2017,37(5):665-675. |
| APA | Jiang, Yuanzhong.,Guo, Li.,Ma, Xiaodong.,Zhao, Xin.,Jiao, Bo.,...&Luo, Keming.(2017).The WRKY transcription factors PtrWRKY18 and PtrWRKY35 promote Melampsora resistance in Populus.tree physiology,37(5),665-675. |
| MLA | Jiang, Yuanzhong,et al."The WRKY transcription factors PtrWRKY18 and PtrWRKY35 promote Melampsora resistance in Populus".tree physiology 37.5(2017):665-675. |
入库方式： OAI收割
来源：西北高原生物研究所
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