Investigation of Calmodulin-Peptide Interactions Using Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry
文献类型：期刊论文
| 作者 | Wang ZF ; Yu XM ; Cui M ; Liu ZQ ; Song FR ; Liu SY |
| 刊名 | journal of the american society for mass spectrometry
 |
| 出版日期 | 2009 |
| 卷号 | 20期号:4页码:576-583 |
| 关键词 | ION-CYCLOTRON RESONANCE MALDI-TOF-MS ELECTROSPRAY-IONIZATION NONCOVALENT COMPLEXES PROTEASE INHIBITORS BIOLOGICAL SAMPLES MELITTIN COMPLEX INFLUENZA-VIRUS ESI-MS BINDING |
| ISSN号 | 1044-0305 |
| 通讯作者 | liu zq |
| 中文摘要 | in this report, matrix-assisted laser desorption/ionization mass spectrometry (maldi-ms) was used to study the binding interactions between calmodulin and two target peptides (melittin and substance p). various matrix conditions were tested and the less acidic matrix dhap and thap were found to favor the survival of the intact calcium-calmodulin as well as the calmodulin-peptide complexes. however, the application of direct maldi-ms to detect the intact complexes turned out to be very difficult due to the dissociation of the complexes and the formation of nonspecific aggregates. in contrast, the specific binding of the target peptides to calmodulin could be easily deduced using intensity-fading (if) maldi-ms. compared with the nonbinding control, clear reduction in the ion abundances of the target peptides was observed with the addition of calmodulin. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000265034300005 |
| 公开日期 | 2010-05-04 |
| 源URL | [http://202.98.16.49/handle/322003/11495]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Wang ZF,Yu XM,Cui M,et al. Investigation of Calmodulin-Peptide Interactions Using Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry[J]. journal of the american society for mass spectrometry,2009,20(4):576-583. |
| APA | Wang ZF,Yu XM,Cui M,Liu ZQ,Song FR,&Liu SY.(2009).Investigation of Calmodulin-Peptide Interactions Using Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry.journal of the american society for mass spectrometry,20(4),576-583. |
| MLA | Wang ZF,et al."Investigation of Calmodulin-Peptide Interactions Using Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry".journal of the american society for mass spectrometry 20.4(2009):576-583. |
入库方式： OAI收割
来源：长春应用化学研究所
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