Multifunctional G-Quadruplex Aptamers and Their Application to Protein Detection
文献类型：期刊论文
| 作者 | Li T ; Shi LL ; Wang EK ; Dong SJ |
| 刊名 | chemistry-a european journal
 |
| 出版日期 | 2009 |
| 卷号 | 15期号:4页码:1036-1042 |
| 关键词 | TETRAD-FORMING OLIGONUCLEOTIDES INTRAMOLECULAR DNA QUADRUPLEXES G-RICH OLIGONUCLEOTIDES CATIONIC PORPHYRINS PEROXIDASE-ACTIVITY ANTIPROLIFERATIVE ACTIVITY HIV-1 INTEGRASE HUMAN THROMBIN IN-VITRO BINDING |
| ISSN号 | 0947-6539 |
| 通讯作者 | dong sj |
| 中文摘要 | two significant g-quadruplex aptamers named agro100 and t30695 are identified as multi functional aptamers that can bind the protein ligands nucleolin or hiv-1 integrase and hemin. besides their strong binding to target proteins, both agro100 and t30695 exhibit high hemin-binding affinities comparable to that of the known aptamer (termed ps2m) selected by the in vitro evolution process. most importantly, their corresponding hemin-dna complexes reveal excellent peroxidase-like activities. higher than that of the reported hemin-ps2m dnazyme. this enables these multifunctional aptamers to be applied to the sensitive detection of proteins. which is demonstrated by applying agro100 to the chemiluminescence detection of nucleolin expressed at the surface of hela cells. based on the specific agro100-nucleolin interaction, the surface-expressed nucleolin of hela cells is labeled in situ with the hemin-agro100 dnazyme, and then determined in the luminol-h2o2 system. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000262886800025 |
| 公开日期 | 2010-05-27 |
| 源URL | [http://202.98.16.49/handle/322003/11907]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Li T,Shi LL,Wang EK,et al. Multifunctional G-Quadruplex Aptamers and Their Application to Protein Detection[J]. chemistry-a european journal,2009,15(4):1036-1042. |
| APA | Li T,Shi LL,Wang EK,&Dong SJ.(2009).Multifunctional G-Quadruplex Aptamers and Their Application to Protein Detection.chemistry-a european journal,15(4),1036-1042. |
| MLA | Li T,et al."Multifunctional G-Quadruplex Aptamers and Their Application to Protein Detection".chemistry-a european journal 15.4(2009):1036-1042. |
入库方式： OAI收割
来源：长春应用化学研究所
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