Comparison of bacterial community characteristics between complete and shortcut denitrification systems for quinoline degradation
文献类型：期刊论文
| 作者 | Zhang, Xiaomei1; Hua, Xiufu2; Yue, Xiuping3 |
| 刊名 | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
 |
| 出版日期 | 2017-02-01 |
| 卷号 | 101期号:4页码:1697-1707 |
| 关键词 | Quinoline degradation Shortcut denitrification Bacterial community PCR-DGGE High-throughput sequencing qPCR |
| 英文摘要 | For quinoline-denitrifying degradation, very few researches focused on shortcut denitrification process and its bacterial community characteristics. In this study, complete and shortcut denitrification systems were constructed simultaneously for quinoline degradation. By calculation, specific quinoline removal rates were 0.905 and 1.123 g/(gVSS d), respectively, in the complete and shortcut systems, and the latter was 1.24 times of the former. Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE), high-throughput sequencing, and quantitative PCR (qPCR) techniques based on 16S rRNA were jointly applied to compare microbial community structures of two systems. Many denitrifying bacteria phyla, classes, and genera were detected in the two systems. Phylum Proteobacteria, Class Gammaproteobacteria, and Genus Alicycliphilus denitrificans were the dominant contributors for quinoline-denitrifying degradation. In the shortcut denitrification system, main and specific strains playing crucial roles were more; the species richness and the total abundance of functional genes (narG, nirS, nirK, and nosZ) were higher compared with the complete denitrification system. It could be supposed that inorganic-nitrogen reductase activity of bacterial community was stronger in the shortcut denitrification system, which was the intrinsic reason to result in higher denitrification rate. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | RIBOSOMAL-RNA GENE ; WASTE-WATER ; MICROBIAL COMMUNITY ; FUNCTIONAL GENES ; HYDROGENOTROPHIC DENITRIFICATION ; CONSTRUCTED WETLAND ; DIVERSITY ; REACTOR ; POPULATIONS ; PCR |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000393687400033 |
| 源URL | [http://ir.qibebt.ac.cn/handle/337004/9321]  |
| 专题 | 青岛生物能源与过程研究所_规划战略与信心中心 |
| 作者单位 | 1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China 2.Tsinghua Univ, Dept Sci Res & Dev, Beijing 100084, Peoples R China 3.Taiyuan Univ Technol, Environm Sci & Engn Inst, 79 Yingze West St, Taiyuan 030024, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Xiaomei,Hua, Xiufu,Yue, Xiuping. Comparison of bacterial community characteristics between complete and shortcut denitrification systems for quinoline degradation[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2017,101(4):1697-1707. |
| APA | Zhang, Xiaomei,Hua, Xiufu,&Yue, Xiuping.(2017).Comparison of bacterial community characteristics between complete and shortcut denitrification systems for quinoline degradation.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,101(4),1697-1707. |
| MLA | Zhang, Xiaomei,et al."Comparison of bacterial community characteristics between complete and shortcut denitrification systems for quinoline degradation".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 101.4(2017):1697-1707. |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
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