DGE-seq analysis of MUR3-related Arabidopsis mutants provides insight into how dysfunctional xyloglucan affects cell elongation
文献类型：期刊论文
| 作者 | Xu, Zongchang1,2; Wang, Meng1,2; Shi, Dachuan1; Zhou, Gongke3 ; Niu, Tiantian1; Hahn, Michael G.4,5; O'Neill, Malcolm A.4; Kong, Yingzhen1
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| 刊名 | PLANT SCIENCE
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| 出版日期 | 2017-05-01 |
| 卷号 | 258页码:156-169 |
| 关键词 | Arabidopsis MUR3 Xyloglucan Cell elongation Hypocotyl DGE-seq |
| 英文摘要 | Our previous study of the Arabidopsis mur3-3 mutant and mutant plants in which the mur3-3 phenotypes are suppressed (xxt2mur3-3, xxt5mur3-3, xxt1xxt2mur3-3 and 35Spro:XLT2:mur3-3) showed that hypocotyl cell elongation is decreased in plants that synthesize galactose-deficient xyloglucan. To obtain genome-wide insight into the transcriptome changes and regulatory networks that may be involved in this decreased elongation, we performed digital gene expression analyses of the etiolated hypocotyls of wild type (WT), mur3-3 and the four suppressor lines. Numerous differentially expressed genes (DEGs) were detected in comparisons between WT and mur3-3 (1423), xxt2mur3-3 and mur3-3 (675), xxt5mur3-3 and mur3-3 (1272), xxt1xxt2mur3-3 and mur3-3 (1197) and 35Spro:XLT2:mur3-3 vs mur3-3 (121). 550 overlapped DEGs were detected among WT vs mur3-3, xxt2mur3-3 vs mur3-3, xxt5mur3-3 vs mur3-3, and xxt1xxt2mur3-3 vs mur3-3 comparisons. These DEGs include 46 cell wall-related genes, 24 transcription factors, 6 hormone-related genes, 9 protein kinase genes and 9 aquaporin genes. The expression of all of the 550 overlapped genes is restored to near wild-type levels in the four mur3-3 suppressor lines. qRT-PCR of fifteen of these 550 genes showed that their expression levels are consistent with the digital gene expression data. Overexpression of some of these genes (XTH4, XTH30, PME3, EXPA11, MYB88, ROT3, AT5G37790, WAG2 and TIP2;3) that are down-regulated in mur3-3 partially rescued the short hypocotyl phenotype but not the aerial phenotype of mur3-3, indicating that different mechanisms exist between hypocotyl cell elongation and leaf cell elongation. (C) 2017 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemistry & Molecular Biology ; Plant Sciences |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Plant Sciences |
| 关键词[WOS] | RNA-SEQ ; PLANT-GROWTH ; METHYL ESTERIFICATION ; FUCOSYL-TRANSFERASE ; ECTOPIC EXPRESSION ; WALL ; THALIANA ; GENE ; BIOSYNTHESIS ; AUXIN |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000399269700015 |
| 源URL | [http://ir.qibebt.ac.cn/handle/337004/9284]  |
| 专题 | 青岛生物能源与过程研究所_植物代谢工程团队 |
| 作者单位 | 1.Chinese Acad Agr Sci, Tobacco Res Inst, Key Lab Tobacco Gene Resources, Qingdao 266101, Peoples R China 2.Chinese Acad Agr Sci, Grad Sch, Beijing 100081, Peoples R China 3.Chinese Acad Sci, Qingdao Engn Res Ctr Biomass Resources & Environm, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China 4.Univ Georgia, Complex Carbohydrate Res Ctr, 220 Riverbend Rd, Athens, GA 30602 USA 5.Univ Georgia, Dept Plant Biol, Athens, GA 30602 USA |
| 推荐引用方式 GB/T 7714 | Xu, Zongchang,Wang, Meng,Shi, Dachuan,et al. DGE-seq analysis of MUR3-related Arabidopsis mutants provides insight into how dysfunctional xyloglucan affects cell elongation[J]. PLANT SCIENCE,2017,258:156-169. |
| APA | Xu, Zongchang.,Wang, Meng.,Shi, Dachuan.,Zhou, Gongke.,Niu, Tiantian.,...&Kong, Yingzhen.(2017).DGE-seq analysis of MUR3-related Arabidopsis mutants provides insight into how dysfunctional xyloglucan affects cell elongation.PLANT SCIENCE,258,156-169. |
| MLA | Xu, Zongchang,et al."DGE-seq analysis of MUR3-related Arabidopsis mutants provides insight into how dysfunctional xyloglucan affects cell elongation".PLANT SCIENCE 258(2017):156-169. |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
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