Dicer and Argonaute Genes Involved in RNA Interference in the Entomopathogenic Fungus Metarhizium robertsii
文献类型：期刊论文
| 作者 | Meng, Huimin1,2; Wang, Zhangxun1,3; Wang, Yulong1; Zhu, Hong1; Huang, Bo1 |
| 刊名 | APPLIED AND ENVIRONMENTAL MICROBIOLOGY
 |
| 出版日期 | 2017-04-01 |
| 卷号 | 83期号:7 |
| 关键词 | Argonaute Dicer Metorhizium robertsii RNA interference |
| 英文摘要 | RNA interference (RNAi) is a gene -silencing mechanism that plays an important role in gene regulation in a number of eukaryotic organisms. Two core components, Dicer and Argonaute, are central in the RNAi machinery. However, the physiological roles of Dicer and Argonaute in the entomopathogenic fungus Metarhizium robertsii have remained unclear. Here, the roles of genes encoding Dicer (M. robertsii dclI1 [Mrdcl1] and Mrdcl2) and Argonaute (Mrago1 and Mrogo2) proteins in M. robertsii were investigated. The results showed that the Dicer -like protein MrDCL2 and Argonaute protein MrAGO1 are the major components of the RNAi process occurring in M. robertsii. The Dicer and Argonaute genes were not involved in the regulation of growth and diverse abiotic stress response in M. robertsii under the tested conditions. Moreover, our results showed that the Dicer and Argonaute gene mutants demonstrated reduced abilities to produce conidia, compared to the wild type (WT) and the gene -rescued mutant. In particular, the conidial yields in the Delta dcl2 and Aogol mutants were reduced by 55.8% and 59.3%, respectively, compared with those from the control strains. Subsequently, for the \ATI and Delta dcl2 mutant strains, digital gene expression (DGE) profiling analysis of the stage of mycelium growth and conidiogenesis revealed that modest changes occur in development or metabolism processes, which may explain the reduction in conidiation in the Delta dcl2 mutant. In addition, we further applied high -throughput sequencing technology to identify small RNAs (sRNAs) that are differentially expressed in the WT and the Delta dcl2 mutant and found that 4 known microRNA-like small RNAs (milRNAs) and 8 novel milRNAs were Mrdcl2 dependent in M. robertsii. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology ; Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology ; Microbiology |
| 关键词[WOS] | GENOME-WIDE IDENTIFICATION ; DOUBLE-STRANDED-RNA ; FILAMENTOUS FUNGI ; ASEXUAL SPORULATION ; SEQUENCE ALIGNMENT ; NEUROSPORA-CRASSA ; STRESS TOLERANCE ; MESSENGER-RNA ; ANISOPLIAE ; EXPRESSION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000397107300016 |
| 源URL | [http://ir.qibebt.ac.cn/handle/337004/9296]  |
| 专题 | 青岛生物能源与过程研究所_微生物资源团队 |
| 作者单位 | 1.Anhui Agr Univ, Anhui Prov Key Lab Microbial Pest Control, Hefei, Peoples R China 2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Prov Key Lab Synthet Biol, Qingdao, Peoples R China 3.Anhui Agr Univ, Sch Plant Protect, Hefei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Meng, Huimin,Wang, Zhangxun,Wang, Yulong,et al. Dicer and Argonaute Genes Involved in RNA Interference in the Entomopathogenic Fungus Metarhizium robertsii[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2017,83(7). |
| APA | Meng, Huimin,Wang, Zhangxun,Wang, Yulong,Zhu, Hong,&Huang, Bo.(2017).Dicer and Argonaute Genes Involved in RNA Interference in the Entomopathogenic Fungus Metarhizium robertsii.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,83(7). |
| MLA | Meng, Huimin,et al."Dicer and Argonaute Genes Involved in RNA Interference in the Entomopathogenic Fungus Metarhizium robertsii".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 83.7(2017). |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
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