Au nanoparticles grafted sandwich platform used amplified small molecule electrochemical aptasensor
文献类型：期刊论文
| 作者 | Du Y ; Li BL ; Wang F ; Dong SJ |
| 刊名 | biosensors & bioelectronics
 |
| 出版日期 | 2009 |
| 卷号 | 24期号:7页码:1979-1983 |
| 关键词 | LABEL-FREE DNA HYBRIDIZATION METHYLENE-BLUE APTAMER GOLD ELECTRODES RECOGNITION SEPARATION BIOSENSORS SELECTION |
| ISSN号 | 0956-5663 |
| 通讯作者 | dong sj |
| 中文摘要 | we report a sensitively amplified electrochemical aptasensor using adenosine triphosphate (atp) as a model. atp is a multifunctional nucleotide thatis most important as a "molecular currency" of intracellular energy transfer. in the sensing process, duplexes consisting of partly complementary strand (pcs1), atp aptamer (aba) and another partly complementary strand (pcs2) were immobilized onto au electrode through the 5'-hs on the pcs1. meanwhile, pcs2 was grafted with the au nanoparticles (aunps) to amplify the detection signals. in the absence of atp, probe methylene blue (mb) bound to the dna duplexes and also bound to guanine bases specifically to produce a strong differential pulse voltammetry (dpv) signal. but when atp exists, the aba-pcs2 or aba-pcs1 part duplexes might be destroyed, which decreased the amount of mb on the electrode and led to obviously decreased dpv signal. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000264791000027 |
| 公开日期 | 2010-05-27 |
| 源URL | [http://202.98.16.49/handle/322003/11925]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Du Y,Li BL,Wang F,et al. Au nanoparticles grafted sandwich platform used amplified small molecule electrochemical aptasensor[J]. biosensors & bioelectronics,2009,24(7):1979-1983. |
| APA | Du Y,Li BL,Wang F,&Dong SJ.(2009).Au nanoparticles grafted sandwich platform used amplified small molecule electrochemical aptasensor.biosensors & bioelectronics,24(7),1979-1983. |
| MLA | Du Y,et al."Au nanoparticles grafted sandwich platform used amplified small molecule electrochemical aptasensor".biosensors & bioelectronics 24.7(2009):1979-1983. |
入库方式： OAI收割
来源：长春应用化学研究所
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