Investigation of 3,3 ',5,5 '-tetramethylbenzidine as colorimetric substrate for a peroxidatic DNAzyme
文献类型：期刊论文
| 作者 | Li BL ; Du Y ; Li T ; Dong SJ |
| 刊名 | analytica chimica acta
 |
| 出版日期 | 2009 |
| 卷号 | 651期号:2页码:234-240 |
| 关键词 | CATALYTIC DNA CLEAVING DNA COMPLEX ENZYME LABEL THROMBIN ASSAY |
| ISSN号 | 0003-2670 |
| 通讯作者 | dong sj |
| 中文摘要 | in this work, the suitability of 3,3',5,5'-tetramethylbenzidine sulfate (tmb) as the substrate of a dnazyme catalytic system composed of a guanine-quadruplex dna molecule and hemin was investigated. in the presence of h2o2, the hemin-dna complex catalyzes the oxidation of tmb to produce two colored products, much like a peroxidase. the color-generating activity of this system could be influenced by several factors such as buffer type, ph value, dna sequence, reaction time, and concentrations of both the hemin and h2o2. to illustrate the utility of this catalytic system, we designed a colorimetric assay, in which a synthetic oligonucleotide with a sequence complementary to the g-quadruplex dna was used as the target. a detection limit of 1.86 nm was obtained. our data have shown that tmb was an excellent colorimetric indicator that reported the peoxidase activities of the widely studied hemin-g-quadruplex dnazyme system. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000270745600015 |
| 公开日期 | 2010-05-27 |
| 源URL | [http://202.98.16.49/handle/322003/12167]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Li BL,Du Y,Li T,et al. Investigation of 3,3 ',5,5 '-tetramethylbenzidine as colorimetric substrate for a peroxidatic DNAzyme[J]. analytica chimica acta,2009,651(2):234-240. |
| APA | Li BL,Du Y,Li T,&Dong SJ.(2009).Investigation of 3,3 ',5,5 '-tetramethylbenzidine as colorimetric substrate for a peroxidatic DNAzyme.analytica chimica acta,651(2),234-240. |
| MLA | Li BL,et al."Investigation of 3,3 ',5,5 '-tetramethylbenzidine as colorimetric substrate for a peroxidatic DNAzyme".analytica chimica acta 651.2(2009):234-240. |
入库方式： OAI收割
来源：长春应用化学研究所
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