Methylene blue as an indicator for sensitive electrochemical detection of adenosine based on aptamer switch
文献类型：期刊论文
| 作者 | Wang JL ; Wang F ; Dong SJ |
| 刊名 | journal of electroanalytical chemistry
 |
| 出版日期 | 2009 |
| 卷号 | 626期号:1-2页码:1-5 |
| 关键词 | LABEL-FREE SIGNALING APTAMERS SMALL MOLECULES BLOOD-SERUM SENSOR ELECTRODE DNA DISPLACEMENT BIOSENSOR COCAINE |
| ISSN号 | 1572-6657 |
| 通讯作者 | dong sj |
| 中文摘要 | in this paper, a simple, label-free and regenerative method was proposed to study the interaction between aptamer and small molecule by using methylene blue (mb+) as an electrochemical indicator. a thiolated capture probe containing twelve bases was firstly self-assembled on gold electrode by gold-sulfur affinity. aptamer probe containing thirty two bases, which was designed to hybridize with capture dna sequence and specifically recognize adenosine, was then immobilized on the electrode surface by hybridization reaction. mb+ was abundantly adsorbed on the aptamer probe by the specific interaction between mb+ and guanine base in aptamer probe. mb+-anchored aptamer probe can be forced to dissociate from the sensing interface after adenosine triggered structure switching of the aptamer. the peak current of mb+ linearly decreased with the concentration of adenosine over a range of 2 x 10 (8)- x 10 (6) m with a detection limit of 1 x 10 (8) m. in addition, we examined the selectivity of this electrochemical biosensor for cytidine, uridine and guanosine that belonged to the nucleosides family and possessed 1 similar structure with adenosine. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000263763500001 |
| 公开日期 | 2010-05-27 |
| 源URL | [http://202.98.16.49/handle/322003/12171]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Wang JL,Wang F,Dong SJ. Methylene blue as an indicator for sensitive electrochemical detection of adenosine based on aptamer switch[J]. journal of electroanalytical chemistry,2009,626(1-2):1-5. |
| APA | Wang JL,Wang F,&Dong SJ.(2009).Methylene blue as an indicator for sensitive electrochemical detection of adenosine based on aptamer switch.journal of electroanalytical chemistry,626(1-2),1-5. |
| MLA | Wang JL,et al."Methylene blue as an indicator for sensitive electrochemical detection of adenosine based on aptamer switch".journal of electroanalytical chemistry 626.1-2(2009):1-5. |
入库方式： OAI收割
来源：长春应用化学研究所
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