The effect of nocodazole on the transfection efficiency of lipid-bilayer coated gold nanoparticles
文献类型：期刊论文
| 作者 | Li D ; Li PC ; Li GP ; Wang J ; Wang EK |
| 刊名 | biomaterials
 |
| 出版日期 | 2009 |
| 卷号 | 30期号:7页码:1382-1388 |
| 关键词 | NONVIRAL GENE-THERAPY CATIONIC LIPOSOMES MEDIATED TRANSFECTION MAMMALIAN-CELLS DNA COMPLEXES DELIVERY ENDOCYTOSIS MICROTUBULES EXPRESSION LIPOPLEXES |
| ISSN号 | 0142-9612 |
| 通讯作者 | wang j |
| 中文摘要 | nonviral vectors are safer than viral systems for gene therapy applications. however, the limited efficacy always prevents their being widely used in clinical practice. aside from searching new gene nonviral vectors, many researchers focus on finding out new substances to improve the transfection efficiency of existent vectors. in this work, we found a transfection enhancer, nocodazole (ncz), for dimethyldioctadecylammonium (dodab, a cationic lipid) bilayer coated gold nanoparticles (aunps) mediated gene delivery. it was found that ncz produces 3-fold transfection enhancement to hek 293t cells assessed by flow cytometry (fcm). the result was further confirmed by luciferase assay, in which ncz induced more than 5 times improvement in transfection efficiency after 48 h of transfection. the results from the inductively coupled plasma mass spectrometry (icp-ms) and fcm showed that ncz did not affect the internalization of dodab-aunps/dna complexes. the trafficking of the complexes by transmission electron microscopy (tem) indicated that the interrupted transportation of the complexes to the lysosomes contributed greatly to the transfection enhancement. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000263017800016 |
| 公开日期 | 2010-05-27 |
| 源URL | [http://202.98.16.49/handle/322003/12189]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Li D,Li PC,Li GP,et al. The effect of nocodazole on the transfection efficiency of lipid-bilayer coated gold nanoparticles[J]. biomaterials,2009,30(7):1382-1388. |
| APA | Li D,Li PC,Li GP,Wang J,&Wang EK.(2009).The effect of nocodazole on the transfection efficiency of lipid-bilayer coated gold nanoparticles.biomaterials,30(7),1382-1388. |
| MLA | Li D,et al."The effect of nocodazole on the transfection efficiency of lipid-bilayer coated gold nanoparticles".biomaterials 30.7(2009):1382-1388. |
入库方式： OAI收割
来源：长春应用化学研究所
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