Anticancer Drug-DNA Interactions Measured Using a Photoinduced Electron-Transfer Mechanism Based on Luminescent Quantum Dots
文献类型：期刊论文
| 作者 | Yuan JP ; Guo WW ; Yang XR ; Wang E |
| 刊名 | analytical chemistry
 |
| 出版日期 | 2009 |
| 卷号 | 81期号:1页码:362-368 |
| 关键词 | RESONANCE ENERGY-TRANSFER STRANDED-DNA MITOXANTRONE BINDING FLUORESCENCE ACID HYBRIDIZATION NANOCRYSTALS DONORS ASSAY |
| ISSN号 | 0003-2700 |
| 通讯作者 | wang e |
| 中文摘要 | a sensing system based on the photoinduced electron transfer of quantum dots (qds) was designed to measure the interaction of anticancer drug and dna, taking mitoxantrone (mtx) as a model drug. mtx adsorbed on the surface of qds can quench the photoluminescence (pl) of qds through the photoinduced electron-transfer process; and then the addition of dna will bring the restoration of qds pl intensity, as dna can bind with mtx and remove it from qds. sensitive detection of mtx with the detection limit of 10 nmol l-1 and a linear detection range from 10 nmol l-1 to 4.5 mu mol l-1 was achieved. the dependence of pl intensity on dna amount was successfully utilized to investigate the interactions between mtx and dna. both the binding constants and the sizes of binding site of mtx-dna interactions were calculated based on the equations deduced for the pl recovery process. the binding constant obtained in our experiment was generally consistent with previous reports. the sensitive and speedy detection of mtx as well as the avoidance of modification or immobilization process made this system suitable and promising in the drug-dna interaction studies. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000262113400051 |
| 公开日期 | 2010-05-27 |
| 源URL | [http://202.98.16.49/handle/322003/12193]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Yuan JP,Guo WW,Yang XR,et al. Anticancer Drug-DNA Interactions Measured Using a Photoinduced Electron-Transfer Mechanism Based on Luminescent Quantum Dots[J]. analytical chemistry,2009,81(1):362-368. |
| APA | Yuan JP,Guo WW,Yang XR,&Wang E.(2009).Anticancer Drug-DNA Interactions Measured Using a Photoinduced Electron-Transfer Mechanism Based on Luminescent Quantum Dots.analytical chemistry,81(1),362-368. |
| MLA | Yuan JP,et al."Anticancer Drug-DNA Interactions Measured Using a Photoinduced Electron-Transfer Mechanism Based on Luminescent Quantum Dots".analytical chemistry 81.1(2009):362-368. |
入库方式： OAI收割
来源：长春应用化学研究所
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