[Ru(bpy)(2)(dcbpy)NHS] Labeling/Aptamer-Based Biosensor for the Detection of Lysozyme by Increasing Sensitivity with Gold Nanoparticle Amplification
文献类型：期刊论文
| 作者 | Bai JG ; Wei H ; Li BL ; Song LH ; Fang LY ; Lv ZZ ; Zhou WH ; Wang EK |
| 刊名 | chemistry-an asian journal
 |
| 出版日期 | 2008 |
| 卷号 | 3期号:11页码:1935–1941 |
| 关键词 | SWITCHING SIGNALING APTAMERS IN-VITRO SELECTION RU(BPY)(3)(3+)-BASED CHEMILUMINESCENCE DETECTION FUNCTIONALIZED AU-NANOPARTICLES ELECTROGENERATED CHEMILUMINESCENCE ELECTROCHEMILUMINESCENCE DETECTION EMISSION INTENSITY SMALL MOLECULES PARTICLE-SIZE RNA MOLECULES |
| ISSN号 | 1861-4728 |
| 通讯作者 | zhou wh |
| 中文摘要 | a novel [ru(bpy)(2) (dcbpy)nhs] labeling/aptamer-based biosensor combined with gold nanoparticle amplification for the determination of lysozyme with an electrochemiluminescence (ecl) method is presented. in this work, an aptamer, an ecl probe, gold nanoparticle amplification, and competition assay are the main protocols employed in ecl detection. with all the protocols used, an original biosensor coupled with an aptamer and [ru(bpy)(2)(dcbpy)nhs] has been prepared. its high selectivity and sensitivity are the main advantages over other traditional [ru(bpy)(3)](2+) biosensors. the electrochemical impedance spectroscopy (eis) and atomic force microscopy (afm) characterization illustrate that this biosensor is fabricated successfully. finally, the biosensor was applied to a displacement assay in different concentrations of lysozyme solution, and an ultrasensitive ecl signal was obtained. the ecl intensity decreased proportionally to the lysozyme concentration over the range 1.0 x 10-(13)-1.0 x 10(-8) mol l-1 with a detection limit of 1.0 x 10(-13) mol l-1. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000261002000007 |
| 公开日期 | 2010-05-27 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/12551]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Bai JG,Wei H,Li BL,et al. [Ru(bpy)(2)(dcbpy)NHS] Labeling/Aptamer-Based Biosensor for the Detection of Lysozyme by Increasing Sensitivity with Gold Nanoparticle Amplification[J]. chemistry-an asian journal,2008,3(11):1935–1941. |
| APA | Bai JG.,Wei H.,Li BL.,Song LH.,Fang LY.,...&Wang EK.(2008).[Ru(bpy)(2)(dcbpy)NHS] Labeling/Aptamer-Based Biosensor for the Detection of Lysozyme by Increasing Sensitivity with Gold Nanoparticle Amplification.chemistry-an asian journal,3(11),1935–1941. |
| MLA | Bai JG,et al."[Ru(bpy)(2)(dcbpy)NHS] Labeling/Aptamer-Based Biosensor for the Detection of Lysozyme by Increasing Sensitivity with Gold Nanoparticle Amplification".chemistry-an asian journal 3.11(2008):1935–1941. |
入库方式： OAI收割
来源：长春应用化学研究所
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