Microarray-based Raman spectroscopic assay for kinase inhibition by gold nanoparticle probes
文献类型：期刊论文
| 作者 | Li T ; Liu DJ ; Wang ZX |
| 刊名 | biosensors & bioelectronics
 |
| 出版日期 | 2009 |
| 卷号 | 24期号:11页码:3335-3339 |
| ISSN号 | 0956-5663 |
| 关键词 | PEPTIDE ARRAYS PROTEIN PHOSPHORYLATION SPECIFICITY PROTEOMICS BINDING DESIGN SITE |
| 通讯作者 | wang zx |
| 中文摘要 | in this paper, a microarray-based surface-enhanced raman spectroscopic (sers) assay for detection of kinase functionality and inhibition has been reported. biotinylated anti-phosphoserinen antibodies mark the phosphorylation and inhibition events and gold nanoparticles are attached to the antibodies by standard avidin-biotin chemistry, followed by silver deposition for sers signal enhancement. the avidin conjugated fluorescein is used as sers probe. the alpha-catalytic subunit of cyclic adenosine 5'-monophosphate (camp) dependent protein kinase (pka), its well known substrate, kemptide, and three inhibitors, h89, ha1077, and kn62 have been chosen here to establish the sers assay. as expected, highly selective inhibition of pka is demonstrated with the inhibitor h89 and the inhibition assay enable to detect kinase inhibition as well as derive ic50 (half maximal inhibitory concentration) plots. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000267577900026 |
| 公开日期 | 2010-06-18 |
| 源URL | [http://202.98.16.49/handle/322003/12631]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Li T,Liu DJ,Wang ZX. Microarray-based Raman spectroscopic assay for kinase inhibition by gold nanoparticle probes[J]. biosensors & bioelectronics,2009,24(11):3335-3339. |
| APA | Li T,Liu DJ,&Wang ZX.(2009).Microarray-based Raman spectroscopic assay for kinase inhibition by gold nanoparticle probes.biosensors & bioelectronics,24(11),3335-3339. |
| MLA | Li T,et al."Microarray-based Raman spectroscopic assay for kinase inhibition by gold nanoparticle probes".biosensors & bioelectronics 24.11(2009):3335-3339. |
入库方式： OAI收割
来源：长春应用化学研究所
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