Functional gold nanoparticles for studying the interaction of lectin with glycosyl complex on living cellular surfaces
文献类型：期刊论文
| 作者 | Wang JN ; Duan TC ; Sun LL ; Liu DJ ; Wang ZX |
| 刊名 | analytical biochemistry
 |
| 出版日期 | 2009 |
| 卷号 | 392期号:1页码:77-82 |
| 关键词 | PROTEIN INTERACTIONS CONCANAVALIN-A RECOGNITION LIGANDS CANCER TOOLS SIZE |
| ISSN号 | 0003-2697 |
| 通讯作者 | wang zx |
| 中文摘要 | in this study. lectin-conjugated gold nanoparticles (gnps) were prepared by standard biotin-streptavidin chemistry. the lectin-conjugated gnps call be used as ail indicator for studying the interaction of lectin with glycosyl complex on living cellular surfaces due to the high affinity of the lectin with saccharides. the interactions of two well-known lectins (ricinus communis agglutinin and concanavalin a) and three different cell lines (hela, 293, and 293t) were selected here to establish this assay. highly binding affinity of r. communis agglutinin with cells was demonstrated by conventional microscopic and uv-visible spectroscopic studies. in addition, the binding process can be inhibited by galactose, giving further proof of the binding mechanism. (c) 2009 elsevier inc. all rights reserved. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000267920600011 |
| 公开日期 | 2010-06-18 |
| 源URL | [http://202.98.16.49/handle/322003/12635]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Wang JN,Duan TC,Sun LL,et al. Functional gold nanoparticles for studying the interaction of lectin with glycosyl complex on living cellular surfaces[J]. analytical biochemistry,2009,392(1):77-82. |
| APA | Wang JN,Duan TC,Sun LL,Liu DJ,&Wang ZX.(2009).Functional gold nanoparticles for studying the interaction of lectin with glycosyl complex on living cellular surfaces.analytical biochemistry,392(1),77-82. |
| MLA | Wang JN,et al."Functional gold nanoparticles for studying the interaction of lectin with glycosyl complex on living cellular surfaces".analytical biochemistry 392.1(2009):77-82. |
入库方式： OAI收割
来源：长春应用化学研究所
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