Label-Free Colorimetric Detection of Aqueous Mercury Ion (Hg2+) Using Hg2+-Modulated G-Quadruplex-Based DNAzymes
文献类型：期刊论文
| 作者 | Li T ; Dong SJ ; Wang E |
| 刊名 | analytical chemistry
 |
| 出版日期 | 2009 |
| 卷号 | 81期号:6页码:2144-2149 |
| 关键词 | FUNCTIONALIZED GOLD NANOPARTICLES SELECTIVE DETECTION PEROXIDASE-ACTIVITY TELOMERASE ACTIVITY AMPLIFIED ANALYSIS CATALYTIC BEACONS OPTICAL-DETECTION METAL-IONS DNA COMPLEX |
| ISSN号 | 0003-2700 |
| 通讯作者 | wang e |
| 中文摘要 | mercury ion (hg2+) is able to specifically bind to the thymine-thymine (t-t) base pair in a dna duplex, thus providing a rationale for dna-based selective detection of hg2+ with various means. in this work, we for the first time utilize the hg2+-mediated t-t base pair to modulate the proper folding of g-quadruplex dnas and inhibit the dnazyme activity, thereby pioneering a facile approach to sense hg2+ with colorimetry. two bimolecular dna g-quadruplexes containing many t residues are adopted here, which function well in low- and high-salt conditions, respectively. these g-quadruplex dnas are able to bind hemin to form the peroxidase-like dnazymes in the folded state. upon addition of hg2+, the proper folding of g-quadruplex dnas is inhibited due to the formation of t-hg2+-t complex. ibis is reflected by the notable change of the soret band of hemin when investigated by using uv-vis absorption spectroscopy. as a result of hg2+ inhibition, a sharp decrease in the catalytic activity toward the h2o2-mediated oxidation of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)diammonium salt (abts) is observed, accompanied by a change in solution color. through this approach, aqueous hg2+ can be detected at 50 nm (10 ppb) with colorimetry in a facile way, with high selectivity against other metal ions. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000264142000015 |
| 公开日期 | 2010-06-18 |
| 源URL | [http://202.98.16.49/handle/322003/12681]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Li T,Dong SJ,Wang E. Label-Free Colorimetric Detection of Aqueous Mercury Ion (Hg2+) Using Hg2+-Modulated G-Quadruplex-Based DNAzymes[J]. analytical chemistry,2009,81(6):2144-2149. |
| APA | Li T,Dong SJ,&Wang E.(2009).Label-Free Colorimetric Detection of Aqueous Mercury Ion (Hg2+) Using Hg2+-Modulated G-Quadruplex-Based DNAzymes.analytical chemistry,81(6),2144-2149. |
| MLA | Li T,et al."Label-Free Colorimetric Detection of Aqueous Mercury Ion (Hg2+) Using Hg2+-Modulated G-Quadruplex-Based DNAzymes".analytical chemistry 81.6(2009):2144-2149. |
入库方式： OAI收割
来源：长春应用化学研究所
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