中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
G-Quadruplex Aptamers with Peroxidase-Like DNAzyme Functions: Which Is the Best and How Does it Work?

文献类型:期刊论文

作者Li T ; Dong SJ ; Wang EK
刊名chemistry-an asian journal
出版日期2009
卷号4期号:6页码:918–922
关键词FREE COLORIMETRIC DETECTION TELOMERASE ACTIVITY CATIONIC PORPHYRINS CATALYTIC DNA COMPLEX HEMIN OLIGONUCLEOTIDE LIGANDS LABEL THROMBIN
ISSN号1861-4728
通讯作者wang ek
中文摘要some g-quadruplex dna aptamers have been found to strongly bind hemin to form dnazymes with peroxidase-like activity. to help determine the most suitable dnazymes and to understand how they work, five previously reported g-quadruplex aptamers were compared for their binding affinity and then the potential catalytic mechanism of their corresponding hemin-g-quadruplex dnazymes was explored. among these aptamers, a g-quadruplex named agro100 was shown to possess the highest hemin-binding affinity and the best dnazyme function. this means that agro100 is the most ideal candidate for dnazyme-based analysis. furthermore, we found the peroxidase-like activity of dnazyme to be primarily dependent on the concentration of h2o2 and independent of that of the peroxidase substrate (that is, 2,2-azino-bis(3-ethytbenzothiazoline-6-sulfonic acid)diammonium salt). accordingly, a reaction mechanism for dnazyme-catalyzed peroxidation is proposed. this study provides new insights into the g-quadruplex-based dnazymes and will help us to further extend their applications in the analytical field.
收录类别SCI
语种英语
WOS记录号WOS:000266929600018
公开日期2010-06-18
源URL[http://202.98.16.49/handle/322003/12689]  
专题长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式
GB/T 7714
Li T,Dong SJ,Wang EK. G-Quadruplex Aptamers with Peroxidase-Like DNAzyme Functions: Which Is the Best and How Does it Work?[J]. chemistry-an asian journal,2009,4(6):918–922.
APA Li T,Dong SJ,&Wang EK.(2009).G-Quadruplex Aptamers with Peroxidase-Like DNAzyme Functions: Which Is the Best and How Does it Work?.chemistry-an asian journal,4(6),918–922.
MLA Li T,et al."G-Quadruplex Aptamers with Peroxidase-Like DNAzyme Functions: Which Is the Best and How Does it Work?".chemistry-an asian journal 4.6(2009):918–922.

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来源:长春应用化学研究所

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