G-Quadruplex Aptamers with Peroxidase-Like DNAzyme Functions: Which Is the Best and How Does it Work?
文献类型：期刊论文
| 作者 | Li T ; Dong SJ ; Wang EK |
| 刊名 | chemistry-an asian journal
 |
| 出版日期 | 2009 |
| 卷号 | 4期号:6页码:918–922 |
| 关键词 | FREE COLORIMETRIC DETECTION TELOMERASE ACTIVITY CATIONIC PORPHYRINS CATALYTIC DNA COMPLEX HEMIN OLIGONUCLEOTIDE LIGANDS LABEL THROMBIN |
| ISSN号 | 1861-4728 |
| 通讯作者 | wang ek |
| 中文摘要 | some g-quadruplex dna aptamers have been found to strongly bind hemin to form dnazymes with peroxidase-like activity. to help determine the most suitable dnazymes and to understand how they work, five previously reported g-quadruplex aptamers were compared for their binding affinity and then the potential catalytic mechanism of their corresponding hemin-g-quadruplex dnazymes was explored. among these aptamers, a g-quadruplex named agro100 was shown to possess the highest hemin-binding affinity and the best dnazyme function. this means that agro100 is the most ideal candidate for dnazyme-based analysis. furthermore, we found the peroxidase-like activity of dnazyme to be primarily dependent on the concentration of h2o2 and independent of that of the peroxidase substrate (that is, 2,2-azino-bis(3-ethytbenzothiazoline-6-sulfonic acid)diammonium salt). accordingly, a reaction mechanism for dnazyme-catalyzed peroxidation is proposed. this study provides new insights into the g-quadruplex-based dnazymes and will help us to further extend their applications in the analytical field. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000266929600018 |
| 公开日期 | 2010-06-18 |
| 源URL | [http://202.98.16.49/handle/322003/12689]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Li T,Dong SJ,Wang EK. G-Quadruplex Aptamers with Peroxidase-Like DNAzyme Functions: Which Is the Best and How Does it Work?[J]. chemistry-an asian journal,2009,4(6):918–922. |
| APA | Li T,Dong SJ,&Wang EK.(2009).G-Quadruplex Aptamers with Peroxidase-Like DNAzyme Functions: Which Is the Best and How Does it Work?.chemistry-an asian journal,4(6),918–922. |
| MLA | Li T,et al."G-Quadruplex Aptamers with Peroxidase-Like DNAzyme Functions: Which Is the Best and How Does it Work?".chemistry-an asian journal 4.6(2009):918–922. |
入库方式： OAI收割
来源：长春应用化学研究所
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