A Simple, Universal Colorimetric Assay for Endonuclease/Methyltransferase Activity and Inhibition Based on an Enzyme-Responsive Nanoparticle System
文献类型：期刊论文
| 作者 | Song GT ; Chen CE ; Ren JS ; Qu XG |
| 刊名 | acs nano
 |
| 出版日期 | 2009 |
| 卷号 | 3期号:5页码:1183-1189 |
| ISSN号 | 1936-0851 |
| 关键词 | FUNCTIONALIZED GOLD NANOPARTICLES DNA METHYLTRANSFERASE INHIBITORS LINKED-IMMUNOSORBENT-ASSAY RESTRICTION ENDONUCLEASES OPTICAL-PROPERTIES METHYLATION CLEAVAGE RECOMBINATION NUCLEASES CANCER |
| 通讯作者 | ren js |
| 中文摘要 | an enzyme responsive nanoparticle system that uses a dna-gold nanoparticle (aunp) assembly as the substrate has been developed for the simple, sensitive, and universal monitoring of restriction endonucleases in real time. this new assay takes advantage of the palindromic recognition sequence of the restriction nucleases and the unique optical properties of aunps and is simpler than the procedure previously described by by xu et al. (angew. chem. int. ed. engl. 2007, 46, 3468-3470). because it involves only one type of ssdna modified aunps, this assay can be directed toward most of the endonucleases by simply changing the recognition sequence found within the linker dna. in addition, the endonuclease activity could be quantitatively analyzed by the value of the reciprocal of hydrolysis half time (t(1/2)(-1). furthermore, our new design could also be applied to the assay of methyltransferase activity since the methylation of dna inhibits its cleavage by the corresponding restriction endonuclease, and thus, this new methodology can be easily adapted to high-throughput screening of methyltransferase inhibitors. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000266323600021 |
| 公开日期 | 2010-06-18 |
| 源URL | [http://202.98.16.49/handle/322003/12729]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Song GT,Chen CE,Ren JS,et al. A Simple, Universal Colorimetric Assay for Endonuclease/Methyltransferase Activity and Inhibition Based on an Enzyme-Responsive Nanoparticle System[J]. acs nano,2009,3(5):1183-1189. |
| APA | Song GT,Chen CE,Ren JS,&Qu XG.(2009).A Simple, Universal Colorimetric Assay for Endonuclease/Methyltransferase Activity and Inhibition Based on an Enzyme-Responsive Nanoparticle System.acs nano,3(5),1183-1189. |
| MLA | Song GT,et al."A Simple, Universal Colorimetric Assay for Endonuclease/Methyltransferase Activity and Inhibition Based on an Enzyme-Responsive Nanoparticle System".acs nano 3.5(2009):1183-1189. |
入库方式： OAI收割
来源：长春应用化学研究所
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