Sweet spot matching: A thin-layer chromatography-based countercurrent solvent system selection strategy
文献类型：期刊论文
| 作者 | Liu, Yang ; Friesen, J. Brent ; Grzelak, Edyta M. ; Fan, Qingfei ; Tang, Ting ; Duric, Kemal ; Jaki, Birgit U. ; McAlpine, James B. ; Franzblaue, Scott G. ; Chen, Shao-Nong ; Pauli, Guido F. |
| 刊名 | JOURNAL OF CHROMATOGRAPHY A
 |
| 出版日期 | 2017 |
| 卷号 | 1504期号:x页码:46-100 |
| 中文摘要 | TLC-based strategies were proposed in 1979 (Hostettmann et al.) and 2005 (Friesen & Pauli; GUESS method) to minimize the number of partitioning experiments required for countercurrent separation (CCS) solvent system selection. As semi-empirical approaches, both proposed that the K values defining the sweet spot of optimal CCS corresponded to a matching Rf value range from the silica gel TLC plate developed in the organic phase of a biphasic or a corresponding monophasic solvent system. Despite their simplicity, there has been an absence of theoretical support and a deficiency of reported experimental evidence. The present study explores the theory required to develop correlations between Rf and K. All theoretical models surmise that the optimal Rf value range should be centered at 0.5. In order to validate the feasibility of the concept of matching Rf and K values, 43 natural products and six solvent system families were investigated. Out of 62 correlations, 45 resulted in matched Rf and K values. Based on this study, practical guidelines for the TLC-based prediction strategy are provided. These approaches will equip CCS users with an updated understanding of how to apply the TLC-based solvent system selection strategy to accelerate a targeted selection of CCS conditions. (C) 2017 Elsevier B.V. All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology ; Chemistry |
| 原文出处 | 10.1016/j.chroma.2017.04.055 |
| 语种 | 英语 |
| 公开日期 | 2017-07-18 |
| 源URL | [http://ir.xtbg.org.cn/handle/353005/10509]  |
| 专题 | 西双版纳热带植物园_其他 |
| 推荐引用方式 GB/T 7714 | Liu, Yang,Friesen, J. Brent,Grzelak, Edyta M.,et al. Sweet spot matching: A thin-layer chromatography-based countercurrent solvent system selection strategy[J]. JOURNAL OF CHROMATOGRAPHY A,2017,1504(x):46-100. |
| APA | Liu, Yang.,Friesen, J. Brent.,Grzelak, Edyta M..,Fan, Qingfei.,Tang, Ting.,...&Pauli, Guido F..(2017).Sweet spot matching: A thin-layer chromatography-based countercurrent solvent system selection strategy.JOURNAL OF CHROMATOGRAPHY A,1504(x),46-100. |
| MLA | Liu, Yang,et al."Sweet spot matching: A thin-layer chromatography-based countercurrent solvent system selection strategy".JOURNAL OF CHROMATOGRAPHY A 1504.x(2017):46-100. |
入库方式： OAI收割
来源：西双版纳热带植物园
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