A New Method for Analysis of Disulfide-Containing Proteins by Matrix-Assisted Laser Desorption Ionization (MALDI) Mass Spectrometry
文献类型：期刊论文
| 作者 | Yang HM ; Liu N ; Qiu XY ; Liu SY |
| 刊名 | journal of the american society for mass spectrometry
 |
| 出版日期 | 2009 |
| 卷号 | 20期号:12页码:2284－2293 |
| 关键词 | IN-SOURCE DECAY TIME-OF-FLIGHT DESORPTION/IONIZATION-TIME CHEMICAL-REACTIONS PARTIAL REDUCTION LINKED PEPTIDES BONDS IDENTIFICATION ACRYLAMIDE FRAGMENTATION |
| ISSN号 | 1044-0305 |
| 通讯作者 | liu sy |
| 中文摘要 | a simple and high-throughput method for the identification of disulfide-containing peptides utilizing peptide-matrix adducts is described. some commonly used matrices in maldi mass spectrometry were found to specifically react with sulfhydryl groups within peptide, thus allowing the observation of the peptide-matrix adduct ion [m + n + n' matrix + h](+) or [m + n + n' matrix + na](+) (n = the number of cysteine residues, n' = 1, 2, ..., n) in maldi mass spectra after chemical reduction of disulfide-linked peptides. among several matrices tested, alpha-cyano-4-hydroxycinnamic acid (chca, molecular mass 189 da) and alpha-cyano-3-hydroxycinnamic acid (3-hcca) were found to be more effective for maldi analysis of disulfide-containing peptides/proteins. two reduced cysteines involved in a disulfide bridge resulted in a mass shift of 189 da per cysteine, so the number of disulfide bonds could then be determined, while for the other matrices (sinapinic acid, ferulic acid, and caffeic acid), a similar addition reaction could not occur unless the reaction was carried out under alkaline conditions. the underlying mechanism of the reaction of the matrix addition at sulfhydryl groups is proposed, and several factors that might affect the formation of the peptide-matrix adducts were investigated. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000272628200014 |
| 公开日期 | 2010-06-18 |
| 源URL | [http://202.98.16.49/handle/322003/12983]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Yang HM,Liu N,Qiu XY,et al. A New Method for Analysis of Disulfide-Containing Proteins by Matrix-Assisted Laser Desorption Ionization (MALDI) Mass Spectrometry[J]. journal of the american society for mass spectrometry,2009,20(12):2284－2293. |
| APA | Yang HM,Liu N,Qiu XY,&Liu SY.(2009).A New Method for Analysis of Disulfide-Containing Proteins by Matrix-Assisted Laser Desorption Ionization (MALDI) Mass Spectrometry.journal of the american society for mass spectrometry,20(12),2284－2293. |
| MLA | Yang HM,et al."A New Method for Analysis of Disulfide-Containing Proteins by Matrix-Assisted Laser Desorption Ionization (MALDI) Mass Spectrometry".journal of the american society for mass spectrometry 20.12(2009):2284－2293. |
入库方式： OAI收割
来源：长春应用化学研究所
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