Multi-armed poly(L-glutamic acid)-graft-oligoethylenimine copolymers as efficient nonviral gene delivery vectors
文献类型：期刊论文
| 作者 | Chen L ; Tian HY ; Chen J ; Chen XS ; Huang YB ; Jing XB |
| 刊名 | journal of gene medicine
 |
| 出版日期 | 2010 |
| 卷号 | 12期号:1页码:64-76 |
| 关键词 | GLUTAMIC ACID-DERIVATIVES PLASMID DNA MULTIFUNCTIONAL VECTORS NCA-POLYMERIZATION BLOCK-COPOLYMER IN-VITRO POLYETHYLENIMINE THERAPY SIRNA TRANSFECTION |
| ISSN号 | 1099-498x |
| 通讯作者 | chen xs |
| 中文摘要 | background the application of polyethylenimine (pei) in gene delivery has been severely limited by significant cytotoxicity that results from a nondegradable methylene backbone and high cationic charge density. it is therefore necessary to develop novel biodegradable pei derivates for low-toxic, highly efficient gene delivery.methods a series of novel cationic copolymers with various charge density were designed and synthesized by grafting different kinds of oligoethylenimine (oei) onto a determinate multi-armed poly(l-glutamic acid) backbone. the molecular structures of multi-armed poly(l-glutamic acid)-graft-oei (mp-g-oei) copolymers were characterized using nuclear magnetic resonance, viscosimetry and gel permeation chromatography. moreover, the mp-g-oei/dna complexes were measured by a gel retardation assay, dynamic light scattering and atomic force microscopy to determine dna binding ability, particle size, zeta potential, complex formation and shape, respectively. mp-g-oei copolymers were also evaluated in chinese hamster ovary and human embryonic kidney-293 cells for their cytotoxicity and transfection efficiency. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000273895600007 |
| 公开日期 | 2010-06-18 |
| 源URL | [http://202.98.16.49/handle/322003/13077]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Chen L,Tian HY,Chen J,et al. Multi-armed poly(L-glutamic acid)-graft-oligoethylenimine copolymers as efficient nonviral gene delivery vectors[J]. journal of gene medicine,2010,12(1):64-76. |
| APA | Chen L,Tian HY,Chen J,Chen XS,Huang YB,&Jing XB.(2010).Multi-armed poly(L-glutamic acid)-graft-oligoethylenimine copolymers as efficient nonviral gene delivery vectors.journal of gene medicine,12(1),64-76. |
| MLA | Chen L,et al."Multi-armed poly(L-glutamic acid)-graft-oligoethylenimine copolymers as efficient nonviral gene delivery vectors".journal of gene medicine 12.1(2010):64-76. |
入库方式： OAI收割
来源：长春应用化学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。