中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Ionic liquids as selectors for the enhanced detection of proteins

文献类型:期刊论文

作者Li T ; Li BL ; Dong SJ ; Wang EK
刊名chemistry-a european journal
出版日期2007
卷号13期号:30页码:8516-8521
关键词AMPLIFIED SAMPLE INJECTION CAPILLARY-ELECTROPHORESIS ELECTROCHEMILUMINESCENCE DETECTION GAS-CHROMATOGRAPHY STATIONARY PHASES NONIONIC SURFACTANT GOLD NANOPARTICLES SEPARATION AMPLIFICATION NANOCRYSTALS
ISSN号0947-6539
通讯作者dong sj
中文摘要herein, we report an approach for protein detection enhanced by ionic liquid (il) selectors in capillary electrophoresis (ce), with avidin as a model protein. hydrophilic ils were added into the running buffer of ce and acted as selectors for sample injection, enriching the positive target and excluding the negative from the capillary. when using 3% (v/v) il selector, the detection sensitivity of avidin was improved by over one order of magnitude, while the interference from protein adsorption was effectively avoided, even in an uncoated capillary. the electrochemiluminescence method was initially used for il-based ce with low noise that was independent of the il concentration, making ils almost transparent as additives in the electrophoresis buffer.
收录类别SCI
语种英语
WOS记录号WOS:000250330500014
公开日期2010-07-13
源URL[http://ir.ciac.jl.cn/handle/322003/13107]  
专题长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式
GB/T 7714
Li T,Li BL,Dong SJ,et al. Ionic liquids as selectors for the enhanced detection of proteins[J]. chemistry-a european journal,2007,13(30):8516-8521.
APA Li T,Li BL,Dong SJ,&Wang EK.(2007).Ionic liquids as selectors for the enhanced detection of proteins.chemistry-a european journal,13(30),8516-8521.
MLA Li T,et al."Ionic liquids as selectors for the enhanced detection of proteins".chemistry-a european journal 13.30(2007):8516-8521.

入库方式: OAI收割

来源:长春应用化学研究所

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