Ionic liquids as selectors for the enhanced detection of proteins
文献类型:期刊论文
作者 | Li T ; Li BL ; Dong SJ ; Wang EK |
刊名 | chemistry-a european journal
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出版日期 | 2007 |
卷号 | 13期号:30页码:8516-8521 |
关键词 | AMPLIFIED SAMPLE INJECTION CAPILLARY-ELECTROPHORESIS ELECTROCHEMILUMINESCENCE DETECTION GAS-CHROMATOGRAPHY STATIONARY PHASES NONIONIC SURFACTANT GOLD NANOPARTICLES SEPARATION AMPLIFICATION NANOCRYSTALS |
ISSN号 | 0947-6539 |
通讯作者 | dong sj |
中文摘要 | herein, we report an approach for protein detection enhanced by ionic liquid (il) selectors in capillary electrophoresis (ce), with avidin as a model protein. hydrophilic ils were added into the running buffer of ce and acted as selectors for sample injection, enriching the positive target and excluding the negative from the capillary. when using 3% (v/v) il selector, the detection sensitivity of avidin was improved by over one order of magnitude, while the interference from protein adsorption was effectively avoided, even in an uncoated capillary. the electrochemiluminescence method was initially used for il-based ce with low noise that was independent of the il concentration, making ils almost transparent as additives in the electrophoresis buffer. |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000250330500014 |
公开日期 | 2010-07-13 |
源URL | [http://ir.ciac.jl.cn/handle/322003/13107] ![]() |
专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
推荐引用方式 GB/T 7714 | Li T,Li BL,Dong SJ,et al. Ionic liquids as selectors for the enhanced detection of proteins[J]. chemistry-a european journal,2007,13(30):8516-8521. |
APA | Li T,Li BL,Dong SJ,&Wang EK.(2007).Ionic liquids as selectors for the enhanced detection of proteins.chemistry-a european journal,13(30),8516-8521. |
MLA | Li T,et al."Ionic liquids as selectors for the enhanced detection of proteins".chemistry-a european journal 13.30(2007):8516-8521. |
入库方式: OAI收割
来源:长春应用化学研究所
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