Label free electrochemiluminescence protocol for sensitive DNA detection with a tris(2,2'-bipyridyl)ruthenium(II) modified electrode based on nucleic acid oxidation
文献类型：期刊论文
| 作者 | Wei H ; Du Y ; Kang JZ ; Wang EK |
| 刊名 | electrochemistry communications
 |
| 出版日期 | 2007 |
| 卷号 | 9期号:7页码:1474-1479 |
| 关键词 | ELECTROGENERATED CHEMILUMINESCENCE GUANINE OXIDATION ULTRATHIN FILMS RU(BPY)(3)(2+) RUTHENIUM(II) NANOPARTICLES HYBRIDIZATION ISOTOPE SOLVENT TARGETS |
| ISSN号 | 1388-2481 |
| 通讯作者 | wang ek |
| 中文摘要 | label free electrochemiluminescence (ecl) dna detection based on catalytic guanine and adenine bases oxidation using tris(2,2'-bipyridyl)ruthenium(ii) [ru(bpy)(3)(2+)] modified glassy carbon (gc) electrode was demonstrated in this work. the modified gc electrode was prepared by casting carbon nanotubes (cnt)/nafion/ru(bpy)(3)(2+) composite film on the electrode surface. ecl signals of doublestranded dna and their thermally denatured counterparts can be distinctly discriminated using cyclic voltammetry (cv) with a low concentration (3.04 x 10(-8) mol/l for salmon testes-dna). most importantly, sensitive single-base mismatch detection of p53 gene sequence segment was realized with 3.93 x 10(-10) mol/l employing cv stimulation (ecl signal of c/a mismatched dna oligonucleotides was 1.5-fold higher than that of fully base-paired dna oligonucleotides). label free, high sensitivity and simplicity for single-base mismatch discrimination were the main advantages of the present ecl technique for dna detection over the traditional dna sensors. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000248089400010 |
| 公开日期 | 2010-07-13 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/13123]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Wei H,Du Y,Kang JZ,et al. Label free electrochemiluminescence protocol for sensitive DNA detection with a tris(2,2'-bipyridyl)ruthenium(II) modified electrode based on nucleic acid oxidation[J]. electrochemistry communications,2007,9(7):1474-1479. |
| APA | Wei H,Du Y,Kang JZ,&Wang EK.(2007).Label free electrochemiluminescence protocol for sensitive DNA detection with a tris(2,2'-bipyridyl)ruthenium(II) modified electrode based on nucleic acid oxidation.electrochemistry communications,9(7),1474-1479. |
| MLA | Wei H,et al."Label free electrochemiluminescence protocol for sensitive DNA detection with a tris(2,2'-bipyridyl)ruthenium(II) modified electrode based on nucleic acid oxidation".electrochemistry communications 9.7(2007):1474-1479. |
入库方式： OAI收割
来源：长春应用化学研究所
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