Adaptive recognition of small molecules by nucleic acid aptamers through a label-free approach
文献类型：期刊论文
| 作者 | Li T ; Li BL ; Dong SJ |
| 刊名 | chemistry-a european journal
 |
| 出版日期 | 2007 |
| 卷号 | 13期号:23页码:6718-6723 |
| 关键词 | PROBE CAPILLARY-ELECTROPHORESIS IN-VITRO SELECTION DNA APTAMERS ELECTROCHEMICAL DETECTION EQUILIBRIUM MIXTURES BIOLOGICAL-FLUIDS COPPER ELECTRODE IONIC LIQUIDS AMINO-ACIDS RNA APTAMER |
| ISSN号 | 0947-6539 |
| 通讯作者 | dong sj |
| 中文摘要 | we report a novel label-free method for the investigation of the adaptive recognition of small molecules by nucleic acid aptamers using capillary electrophoresis analysis. cocaine and argininamide were chosen as model molecules, and the two corresponding dna aptamers were used. these single-strand dnas folded into their specific secondary structures, which were mainly responsible for the binding of the target molecules with high affinity and specificity. for molecular recognition, the nucleic acid structures then underwent additional conformational changes, while keeping the target molecules stabilized by intermolecular hydrogen bonds. the intrinsic chemical and physical properties of the target molecules enabled them to act as indicators for adaptive binding. thus any labeling or modification of the aptamers or target molecules were made obsolete. this label-free method for aptamer-based molecular recognition was also successfully applied to biological fluids and therefore indicates that this approach is a promising tool for bioanalysis. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000248710700021 |
| 公开日期 | 2010-07-13 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/13255]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Li T,Li BL,Dong SJ. Adaptive recognition of small molecules by nucleic acid aptamers through a label-free approach[J]. chemistry-a european journal,2007,13(23):6718-6723. |
| APA | Li T,Li BL,&Dong SJ.(2007).Adaptive recognition of small molecules by nucleic acid aptamers through a label-free approach.chemistry-a european journal,13(23),6718-6723. |
| MLA | Li T,et al."Adaptive recognition of small molecules by nucleic acid aptamers through a label-free approach".chemistry-a european journal 13.23(2007):6718-6723. |
入库方式： OAI收割
来源：长春应用化学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。