Lung alveolar proteomics of bronchoalveolar lavage from a pulmonary alveolar proteinosis patient using high-resolution FTICR mass spectrometry
文献类型：期刊论文
| 作者 | Bai Y ; Galetskiy D ; Damoc E ; Ripper J ; Woischnik M ; Griese M ; Liu ZQ ; Liu SY ; Przybylski M |
| 刊名 | analytical and bioanalytical chemistry
 |
| 出版日期 | 2007 |
| 卷号 | 389期号:4页码:1075-1085 |
| ISSN号 | 1618-2642 |
| 关键词 | ASSISTED-LASER-DESORPTION/IONIZATION N-GLYCOSYLATION SITES CYSTIC-FIBROSIS LIQUID-CHROMATOGRAPHY POLYACRYLAMIDE GELS MALDI-TOF FLUID DISEASE IDENTIFICATION GENE |
| 通讯作者 | przybylski m |
| 中文摘要 | high-resolution fourier transform ion cyclotron resonance (fticr) mass spectrometry was developed and applied to the proteome analysis of bronchoalveolar lavage fluid (balf) from a patient with pulmonary alveolar proteinosis. with use of 1-d and 2-d gel electrophoresis, surfactant protein a (sp-a) and other surfactant-related lung alveolar proteins were efficiently separated and identified by matrix-assisted laser desorption/ionization fticr mass spectrometry . low molecular mass balf proteins were separated using a gradient 2-d gel. an efficient extraction/precipitation system was developed and used for the enrichment of surfactant proteins. the result of the balf proteome analysis show the presence of several isoforms of sp-a, in which an n-non-glycosylierte form and several proline hydroxylations were identified. furthermore, a number of protein spots were found to contain a mixture of proteins unresolved by 2-d gel electrophoresis, illustrating the feasibility of high-resolution mass spectrometry to provide identifications of proteins that remain unseparated in 2-d gels even upon extended ph gradients. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000249729400009 |
| 公开日期 | 2010-07-13 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/13295]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Bai Y,Galetskiy D,Damoc E,et al. Lung alveolar proteomics of bronchoalveolar lavage from a pulmonary alveolar proteinosis patient using high-resolution FTICR mass spectrometry[J]. analytical and bioanalytical chemistry,2007,389(4):1075-1085. |
| APA | Bai Y.,Galetskiy D.,Damoc E.,Ripper J.,Woischnik M.,...&Przybylski M.(2007).Lung alveolar proteomics of bronchoalveolar lavage from a pulmonary alveolar proteinosis patient using high-resolution FTICR mass spectrometry.analytical and bioanalytical chemistry,389(4),1075-1085. |
| MLA | Bai Y,et al."Lung alveolar proteomics of bronchoalveolar lavage from a pulmonary alveolar proteinosis patient using high-resolution FTICR mass spectrometry".analytical and bioanalytical chemistry 389.4(2007):1075-1085. |
入库方式： OAI收割
来源：长春应用化学研究所
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