中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
A novel alcohol dehydrogenase biosensor based on solid-state electrogenerated chemiluminescence by assembling dehydrogenase to Ru(bpy)(3)(2+)-Au nanoparticles aggregates

文献类型:期刊论文

作者Zhang LH ; Xu ZA ; Sun XP ; Dong SJ
刊名biosensors & bioelectronics
出版日期2007
卷号22期号:6页码:1097-1100
关键词ELECTROCHEMILUMINESCENCE DETECTOR CAPILLARY-ELECTROPHORESIS AMPEROMETRIC BIOSENSOR TRIS(2 ETHANOL QUANTITATION OXIDE 2'-BIPYRIDYL)RUTHENIUM(II)
ISSN号0956-5663
通讯作者dong sj
中文摘要based on electrogenerated chemiluminescence (ecl), a novel method for fabrication of alcohol dehydrogenase (adh) biosensor by self-assembling adh to ru(bpy)(3)(2+) -aunps aggregates (ru-aunps) on indium tin oxide (ito) electrode surface has been developed. positively charged ru(bpy)(3)(2+) could be immobilized stably on the electrode surface with negatively charged aunps in the form of aggregate via electrostatic interaction. on the other hand, aunps are favourable candidates for the immobilization of enzymes because amine groups and cysteine residues in the enzymes are known to bind strongly with aunps. moreover, aunps can act as tiny conduction centers to facilitate the transfer of electrons. such biosensor combined enzymatic selectivity with the sensitivity of ecl detection for quantification of enzyme substrate, and it displayed wide linear range, high sensitivity and good stability.
收录类别SCI
语种英语
WOS记录号WOS:000243541800046
公开日期2010-07-13
源URL[http://ir.ciac.jl.cn/handle/322003/13299]  
专题长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式
GB/T 7714
Zhang LH,Xu ZA,Sun XP,et al. A novel alcohol dehydrogenase biosensor based on solid-state electrogenerated chemiluminescence by assembling dehydrogenase to Ru(bpy)(3)(2+)-Au nanoparticles aggregates[J]. biosensors & bioelectronics,2007,22(6):1097-1100.
APA Zhang LH,Xu ZA,Sun XP,&Dong SJ.(2007).A novel alcohol dehydrogenase biosensor based on solid-state electrogenerated chemiluminescence by assembling dehydrogenase to Ru(bpy)(3)(2+)-Au nanoparticles aggregates.biosensors & bioelectronics,22(6),1097-1100.
MLA Zhang LH,et al."A novel alcohol dehydrogenase biosensor based on solid-state electrogenerated chemiluminescence by assembling dehydrogenase to Ru(bpy)(3)(2+)-Au nanoparticles aggregates".biosensors & bioelectronics 22.6(2007):1097-1100.

入库方式: OAI收割

来源:长春应用化学研究所

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