CE with electrochemical detection for investigation of label-free recognition of amino acid amides by guanine-rich DNA aptamers
文献类型：期刊论文
| 作者 | Li T ; Du Y ; Li BL ; Dong SJ |
| 刊名 | electrophoresis
 |
| 出版日期 | 2007 |
| 卷号 | 28期号:17页码:3122-3128 |
| 关键词 | CAPILLARY ZONE ELECTROPHORESIS PULSED AMPEROMETRIC DETECTION LIQUID-CHROMATOGRAPHY MOLECULAR RECOGNITION COPPER ELECTRODE NANOPARTICLES COCAINE SENSOR MICROELECTRODE SEPARATION |
| ISSN号 | 0173-0835 |
| 通讯作者 | dong sj |
| 中文摘要 | in this work, we report a simple and effective investigation into adaptive interactions between guanine-rich dna aptamers and amino acid amides by ce with electrochemical (ec) detection. argininamide (arm) and tyrosinamide (tym) were chosen as model molecules. on a copper electrode, arm generated a good ec signal in 60 mm naoh at 0.7 v (vs ag/ agcl), while tym. was detected well on a platinum electrode at 1. 3 v in 20 mm phosphate of ph 7.0. based on their ec properties, the ligands themselves were used as indicators for the adaptive interactions investigated by ce-ec, making any step of labeling and/or modification of aptamers with indicators exempted. hydrophilic ionic liquid was used as an additive in running buffer of ce to improve the sensitivity of arm detection, whereas the additive was not used for tym. detection due to its negative effect. two guanine-rich dna aptamers were used for molecular recognition of arm and tym. when the aptamers were incubated with ligands, they bound the model molecules with high affinity and specificity, reflected by obvious decreases in the signals of ligands but no changes in those of the control molecules. however, the ligands were hardly affected by the control ssdnas after incubation. the results revealed the specific recognition of arm and tym. by the aptamers. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000249743500021 |
| 公开日期 | 2010-07-13 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/13365]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Li T,Du Y,Li BL,et al. CE with electrochemical detection for investigation of label-free recognition of amino acid amides by guanine-rich DNA aptamers[J]. electrophoresis,2007,28(17):3122-3128. |
| APA | Li T,Du Y,Li BL,&Dong SJ.(2007).CE with electrochemical detection for investigation of label-free recognition of amino acid amides by guanine-rich DNA aptamers.electrophoresis,28(17),3122-3128. |
| MLA | Li T,et al."CE with electrochemical detection for investigation of label-free recognition of amino acid amides by guanine-rich DNA aptamers".electrophoresis 28.17(2007):3122-3128. |
入库方式： OAI收割
来源：长春应用化学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。