Influence of exposed magnetic nanoparticles and their application in chemiluminescence immunoassay
文献类型：期刊论文
| 作者 | Zhang, Zhi1; Guan, Yueping1; Xia, Tingting2; Du, Jiao1; Li, Tao1; Sun, Zongying1; Guo, Chen2 |
| 刊名 | COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
 |
| 出版日期 | 2017-05-05 |
| 卷号 | 520页码:335-342 |
| 关键词 | Magnetic nanoparticles Chemiluminescence Immunoassay Deviation |
| ISSN号 | 0927-7757 |
| 英文摘要 | To a certain extent, uneven coating of nanoparticles may lead to exposure of their contents. In this work, a simulation system was established and exposed Fe3O4 magnetic nanoparticles (MNPs) were used to investigate the influence of the exposed case in a chemiluminescence immunoassay. Some factors such as the concentration of MNPs, coexistence of horseradish peroxidase (HRP), different particle sizes, and various treatment methods were investigated. It was confirmed that both the bodies of nanoparticles and metallic oxides on the exposed surface have associations with the detection signal. This conclusion may provide a new way to explain the deviation emerged in a chemiluminescence immunoassay. In addition, an exposed Fe3O4 MNPs based chemiluminescence immunoassay was developed to detect human chorionic gonadotropin (hCG). Carboxyl-functionalized MNPs were modified with anti-alpha-hCG antibody, and further used as solid-phase supports for sandwich-type immunoassay. The detection limit for hCG under optimal conditions was as low as 1.184 ng/mL with a linear response range of 2-100 ng/mL and a correlation coefficient of 0.989. The proposed method could be used for the determination of hCG in serum samples, which demonstrated their application potential in rapid and cost-effective detection of biological samples. (C) 2017 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Physical Sciences |
| 类目[WOS] | Chemistry, Physical |
| 研究领域[WOS] | Chemistry |
| 关键词[WOS] | IRON-OXIDE NANOPARTICLES ; PEROXIDASE-LIKE ACTIVITY ; ENZYME-IMMUNOASSAY ; SHELL ; QUANTIFICATION ; PERFORMANCE ; SEPARATION ; SERUM |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000401401100038 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/22527]  |
| 专题 | 过程工程研究所_生化工程国家重点实验室 |
| 作者单位 | 1.Univ Sci & Technol Beijing, Sch Mat Sci & Engn, Beijing 100083, Peoples R China 2.Chinese Acad Sci, Inst Proc Engn, Lab Separat Sci & Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Zhi,Guan, Yueping,Xia, Tingting,et al. Influence of exposed magnetic nanoparticles and their application in chemiluminescence immunoassay[J]. COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,2017,520:335-342. |
| APA | Zhang, Zhi.,Guan, Yueping.,Xia, Tingting.,Du, Jiao.,Li, Tao.,...&Guo, Chen.(2017).Influence of exposed magnetic nanoparticles and their application in chemiluminescence immunoassay.COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS,520,335-342. |
| MLA | Zhang, Zhi,et al."Influence of exposed magnetic nanoparticles and their application in chemiluminescence immunoassay".COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS 520(2017):335-342. |
入库方式： OAI收割
来源：过程工程研究所
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