Protective effect of polysaccharide from maca (Lepidium meyenii) on Hep-G2 cells and alcoholic liver oxidative injury in mice
文献类型：期刊论文
| 作者 | Zhang, Lijun1,2; Zhao, Qingsheng1; Wang, Liwei1; Zhao, Mingxia1; Zhao, Bing1 |
| 刊名 | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
 |
| 出版日期 | 2017-06-01 |
| 卷号 | 99页码:63-70 |
| 关键词 | Lepidium meyenii Polysaccharide Alcoholic liver oxidative injury Liver protective effect |
| ISSN号 | 0141-8130 |
| 英文摘要 | To study the characterization and hepatoprotective activity of polysaccharide from maca (Lepidium meyenii), the main polysaccharide from maca (MP-1) was obtained by DEAE-52 cellulose column. The average molecular weight of MP-1 was 1067.3 kDa and the polysaccharide purity was 91.63%. In order to assess the antioxidant activities of MP-1, four kinds of methods were used, including scavenging hydroxyl radical, DPPH, superoxide anion radical, and FRAP, and the results indicated high antioxidant activities. Furthermore, hepatoprotective activity of MP-1 was studied both in vitro and vivo. In vitro, the alcohol induced Hep-G2 cells model was established to evaluate the protective effect of MP-1, which demonstrated MP-1 can alleviate alcohol damage in Hep-G2 cells. In vivo, the Institute of Cancer Researcch (ICR) mice were used to evaluate hepatoprotecive effects of MP-1 on alcoholic liver disease (ALD). Supplement with MP-1 supressed the triglyceride level both in serum and in hepatic tissue. In addition, MP-1 ameliorated serous transaminases increase induced by alcohol, including aspartate transaminase, alanine aminotransferase, and gamma-glutamyl transpeptidase. Moreover, MP-1 also dramatically increased the superoxide dismutase, glutathione peroxidase, and glutathione s-transferase levels in alcoholic mice. Meantime, histopathologic results MP-1 lighten inflammation induced by alcohol. These results indicate that MP-1 possesses hepatoprotective activity against hepatic injury induced by alcohol. (C) 2017 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 类目[WOS] | Biochemistry & Molecular Biology ; Chemistry, Applied ; Polymer Science |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Chemistry ; Polymer Science |
| 关键词[WOS] | ANTIOXIDANT ACTIVITY ; DISEASE ; EXTRACT ; RATS ; SUPPLEMENTATION ; CAPACITY ; BURDEN |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000400034100008 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/22831]  |
| 专题 | 过程工程研究所_生化工程国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Div Biorefineiy Engn, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Lijun,Zhao, Qingsheng,Wang, Liwei,et al. Protective effect of polysaccharide from maca (Lepidium meyenii) on Hep-G2 cells and alcoholic liver oxidative injury in mice[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2017,99:63-70. |
| APA | Zhang, Lijun,Zhao, Qingsheng,Wang, Liwei,Zhao, Mingxia,&Zhao, Bing.(2017).Protective effect of polysaccharide from maca (Lepidium meyenii) on Hep-G2 cells and alcoholic liver oxidative injury in mice.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,99,63-70. |
| MLA | Zhang, Lijun,et al."Protective effect of polysaccharide from maca (Lepidium meyenii) on Hep-G2 cells and alcoholic liver oxidative injury in mice".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 99(2017):63-70. |
入库方式： OAI收割
来源：过程工程研究所
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