pH-dependent protein conformational changes in albumin : gold nanoparticle bioconjugates: A spectroscopic study
文献类型：期刊论文
| 作者 | Shang L ; Wang YZ ; Jiang JG ; Dong SJ |
| 刊名 | langmuir
 |
| 出版日期 | 2007 |
| 卷号 | 23期号:5页码:2714-2721 |
| 关键词 | BOVINE SERUM-ALBUMIN TRANSFORM INFRARED-SPECTROSCOPY CIRCULAR-DICHROISM ENZYMATIC-ACTIVITY COLLOIDAL GOLD SECONDARY STRUCTURE CYTOCHROME-C ADSORPTION FLUORESCENCE PARTICLES |
| ISSN号 | 0743-7463 |
| 通讯作者 | dong sj |
| 中文摘要 | the conformational changes of bovine serum albumin (bsa) in the albumin:gold nanoparticle bioconjugates were investigated in detail by various spectroscopic techniques including uv-vis absorption, fluorescence, circular dichroism, and fourier transform infrared spectroscopies. our studies suggested that albumin in the bioconjugates that was prepared by the common adsorption method underwent substantial conformational changes at both secondary and tertiary structure levels. bsa was found to adopt a more flexible conformational state on the boundary surface of gold nanoparticles as a result of the conformational changes in the bioconjugates. the conformational changes at ph 3.8, 7.0, and 9.0, which corresponded to different isomeric forms of albumin, were investigated, respectively, to probe the ph effect on the conformational changes of bsa in the bioconjugates. the results showed that the ph of the medium influenced the changes greatly and that fluorescence and circular dichroism studies further indicated that the changes were larger at higher ph. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000244248700067 |
| 公开日期 | 2010-07-13 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/13405]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Shang L,Wang YZ,Jiang JG,et al. pH-dependent protein conformational changes in albumin : gold nanoparticle bioconjugates: A spectroscopic study[J]. langmuir,2007,23(5):2714-2721. |
| APA | Shang L,Wang YZ,Jiang JG,&Dong SJ.(2007).pH-dependent protein conformational changes in albumin : gold nanoparticle bioconjugates: A spectroscopic study.langmuir,23(5),2714-2721. |
| MLA | Shang L,et al."pH-dependent protein conformational changes in albumin : gold nanoparticle bioconjugates: A spectroscopic study".langmuir 23.5(2007):2714-2721. |
入库方式： OAI收割
来源：长春应用化学研究所
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