Kinetic study of DNA/DNA hybridization with electrochemical impedance spectroscopy
文献类型：期刊论文
| 作者 | Li DL ; Zou XQ ; Shen Q ; Dong SJ |
| 刊名 | electrochemistry communications
 |
| 出版日期 | 2007 |
| 卷号 | 9期号:2页码:191-196 |
| 关键词 | PLASMON FLUORESCENCE SPECTROSCOPY DNA HYBRIDIZATION LABEL-FREE MONOLAYERS RESONANCE SURFACES DESIGN SENSOR GOLD |
| ISSN号 | 1388-2481 |
| 通讯作者 | dong sj |
| 中文摘要 | the hybridization of immobilized oligonucleotides probe strands with solution phase targets is the underlying principle of microarraybased techniques for the analysis of dna variation. to study the kinetics of dna/dna hybridization, target dna is often prior labeled with markers. a label-free method of electrochemical impedance spectra (eis) for study the hybridization in process was reported. the langmuir model was used to determine the association rate constant (k-on), the dissociation rate constant (k-off) and the affinity rate constant (k-a), for perfect matched dna hybridization. the results show that, eis is a successful technique possessing high effectivity and sensitivity to study dna/dna hybridization kinetics. this work can provide another view on eis for the studying of dna/dna hybridization. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000244587800002 |
| 公开日期 | 2010-07-13 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/13439]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Li DL,Zou XQ,Shen Q,et al. Kinetic study of DNA/DNA hybridization with electrochemical impedance spectroscopy[J]. electrochemistry communications,2007,9(2):191-196. |
| APA | Li DL,Zou XQ,Shen Q,&Dong SJ.(2007).Kinetic study of DNA/DNA hybridization with electrochemical impedance spectroscopy.electrochemistry communications,9(2),191-196. |
| MLA | Li DL,et al."Kinetic study of DNA/DNA hybridization with electrochemical impedance spectroscopy".electrochemistry communications 9.2(2007):191-196. |
入库方式： OAI收割
来源：长春应用化学研究所
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