Aptamer-based label-free method for hemin recognition and DNA assay by capillary electrophoresis with chemiluminescence detection
文献类型：期刊论文
| 作者 | Li T ; Li BL ; Dong SJ |
| 刊名 | analytical and bioanalytical chemistry
 |
| 出版日期 | 2007 |
| 卷号 | 389期号:3页码:887-893 |
| 关键词 | FUNCTIONALIZED AU NANOPARTICLES PEROXIDASE-ACTIVITY TELOMERASE ACTIVITY IMMUNOASSAY PROTEINS DNAZYME COMPLEX CELLS |
| ISSN号 | 1618-2642 |
| 通讯作者 | dong sj |
| 中文摘要 | an aptamer-based label-free approach to hemin recognition and dna assay using capillary electrophoresis with chemiluminescence detection is introduced here. two guanine-rich dna aptamers were used as the recognition element and target dna, respectively. in the presence of potassium ions, the two aptamers folded into the g-quartet structures, binding hemin with high specificity and affinity. based on the g-quartet-hemin interactions, the ligand molecule was specifically recognized with a k (d)approximate to 73 nm, and the target dna could be detected at 0.1 mu m. in phosphate buffer of ph 11.0, hemin catalyzed the h2o2-mediated oxidation of luminol to generate strong chemiluminescence signal; thus the target molecule itself served as an indicator for the molecule-aptamer interaction, which made the labeling and/or modification of aptamers or target molecules unnecessary. this label-free method for molecular recognition and dna detection is therefore simple, easy, and effective. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000249645800025 |
| 公开日期 | 2010-07-13 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/13489]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Li T,Li BL,Dong SJ. Aptamer-based label-free method for hemin recognition and DNA assay by capillary electrophoresis with chemiluminescence detection[J]. analytical and bioanalytical chemistry,2007,389(3):887-893. |
| APA | Li T,Li BL,&Dong SJ.(2007).Aptamer-based label-free method for hemin recognition and DNA assay by capillary electrophoresis with chemiluminescence detection.analytical and bioanalytical chemistry,389(3),887-893. |
| MLA | Li T,et al."Aptamer-based label-free method for hemin recognition and DNA assay by capillary electrophoresis with chemiluminescence detection".analytical and bioanalytical chemistry 389.3(2007):887-893. |
入库方式： OAI收割
来源：长春应用化学研究所
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