Microarray-based kinase inhibition assay by gold nanoparticle probes
文献类型：期刊论文
| 作者 | Sun LL ; Liu DJ ; Wang ZX |
| 刊名 | analytical chemistry
 |
| 出版日期 | 2007 |
| 卷号 | 79期号:2页码:773-777 |
| 关键词 | ULTRASENSITIVE DETECTION PEPTIDE ARRAYS PROTEINS NANOSTRUCTURES SIZE |
| ISSN号 | 0003-2700 |
| 通讯作者 | wang zx |
| 中文摘要 | we report on the development of a new class of kinase microarray for the detection of kinase inhibition based on marking peptide phosphorylation/biotinylation events by attachment of gold nanoparticles followed by silver deposition for signal enhancement. the alpha-catalytic subunit of cyclic adenosine 5'-monophosphate-dependent protein kinase (pka), and its well-known substrate, kemptide, were used for the purpose of monitoring phosphorylation and inhibition. as expected, highly selective inhibition of pka is demonstrated with the four inhibitors: h89, ha1077, mallotoxin, and kn62. furthermore, an inhibition assay demonstrates the ability to detect kinase inhibition as well as derive ic50 (half-maximal inhibitory concentration) plots. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000243415800050 |
| 公开日期 | 2010-07-13 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/13505]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Sun LL,Liu DJ,Wang ZX. Microarray-based kinase inhibition assay by gold nanoparticle probes[J]. analytical chemistry,2007,79(2):773-777. |
| APA | Sun LL,Liu DJ,&Wang ZX.(2007).Microarray-based kinase inhibition assay by gold nanoparticle probes.analytical chemistry,79(2),773-777. |
| MLA | Sun LL,et al."Microarray-based kinase inhibition assay by gold nanoparticle probes".analytical chemistry 79.2(2007):773-777. |
入库方式： OAI收割
来源：长春应用化学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。