A third-generation H2O2 biosensor based on horseradish peroxidase-labeled Au nanoparticles self-assembled to hollow porous polymeric nanopheres
文献类型:期刊论文
作者 | Xu SY ; Peng B ; Han XZ |
刊名 | biosensors & bioelectronics
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出版日期 | 2007 |
卷号 | 22期号:8页码:1807-1810 |
关键词 | DIRECT ELECTRON-TRANSFER GOLD-MODIFIED ELECTRODE SOL-GEL NETWORK HYDROGEN-PEROXIDE AMPEROMETRIC BIOSENSOR ELECTROCHEMISTRY CONSTRUCT MATRIX FILM |
ISSN号 | 0956-5663 |
通讯作者 | xu sy |
中文摘要 | a novel third-generation biosensor for hydrogen peroxide (h2o2) was developed by self-assembling gold nanoparticles to hollow porous thiol-functionalized poly(divinylbenzene-co-acrylic acid) (dvb-co-aa) nanospheres. at first, a cleaned gold electrode was immersed in hollow porous thiol-functionalized poly(dvb-co-aa) nanosphere latex to assemble the nanospheres, then gold nanoparticles were chemisorbed onto the thiol groups of the nanospheres. finally, horseradish peroxidase (hrp) was immobilized on the surface of the gold nanoparticles. the immobilized horseradish peroxidase exhibited direct electrochemical behavior toward the reduction of hydrogen peroxide. the resulting biosensor showed a wide linear range of 1.0 mu m-8.0 mm and a detection limit of 0.5 mu m estimated at a signal-to-noise ratio of 3. moreover, the studied biosensor exhibited high sensitivity, good reproducibility, and long-term stability. |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000244676300033 |
公开日期 | 2010-07-13 |
源URL | [http://ir.ciac.jl.cn/handle/322003/13849] ![]() |
专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
推荐引用方式 GB/T 7714 | Xu SY,Peng B,Han XZ. A third-generation H2O2 biosensor based on horseradish peroxidase-labeled Au nanoparticles self-assembled to hollow porous polymeric nanopheres[J]. biosensors & bioelectronics,2007,22(8):1807-1810. |
APA | Xu SY,Peng B,&Han XZ.(2007).A third-generation H2O2 biosensor based on horseradish peroxidase-labeled Au nanoparticles self-assembled to hollow porous polymeric nanopheres.biosensors & bioelectronics,22(8),1807-1810. |
MLA | Xu SY,et al."A third-generation H2O2 biosensor based on horseradish peroxidase-labeled Au nanoparticles self-assembled to hollow porous polymeric nanopheres".biosensors & bioelectronics 22.8(2007):1807-1810. |
入库方式: OAI收割
来源:长春应用化学研究所
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