Energetic basis of molecular recognition in a DNA aptamer
文献类型：期刊论文
| 作者 | Bishop GR ; Ren JS ; Polander BC ; Jeanfreau BD ; Trent JO ; Chaires JB |
| 刊名 | biophysical chemistry
 |
| 出版日期 | 2007 |
| 卷号 | 126期号:1-3页码:165-175 |
| 关键词 | NUCLEIC-ACID APTAMERS ARGININAMIDE COMPLEX AMINO-ACID BINDING QUADRUPLEX STABILITY EVOLUTION THROMBIN HEAT FOLD |
| ISSN号 | 0301-4622 |
| 通讯作者 | bishop gr |
| 中文摘要 | the thermal stability and ligand binding properties of the l-argininamide-binding dna aptamer (5'-gatcgaaacgtagcgccttcgatc3') were studied by spectroscopic and calorimetric methods. differential calorimetric studies showed that the uncomplexed aptamer melted in a two-state reaction with a melting temperature t-m = 50.2 +/- 0.2 degrees c and a folding enthalpy delta h degrees(fold) = -49.0 +/- 2.1 kcal mol(-1). these values agree with values of t-m = 49.6 degrees c and delta h degrees(fold) = -51.2 kcal mol(-1) predicted for a simple hairpin structure. melting of the uncomplexed aptamer was dependent upon salt concentration, but independent of strand concentration. the t of aptamer melting was found to increase as l-argininamide concentrations increased. analysis of circular dichroism titration data using a single-site binding model resulted in the determination of a binding free energy delta g degrees(bind) = -5.1 kcal mol(-1). isothermal titration calorimetry studies revealed an exothermic binding reaction with delta h degrees(bind) = -8.7 kcal mol(-1). combination of enthalpy and free energy produce ail unfavorable entropy of -t delta s degrees = +3.6 kcal mol(-1). a molar heat capacity change of -116 cal mol(-1) k-1 was determined from calorimetric measurements at four temperatures over the range of 15-40 degrees c. molecular dynamics simulations were used to explore the structures of the unligated and ligated aptamer structures. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000244554000022 |
| 公开日期 | 2010-07-13 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/14085]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Bishop GR,Ren JS,Polander BC,et al. Energetic basis of molecular recognition in a DNA aptamer[J]. biophysical chemistry,2007,126(1-3):165-175. |
| APA | Bishop GR,Ren JS,Polander BC,Jeanfreau BD,Trent JO,&Chaires JB.(2007).Energetic basis of molecular recognition in a DNA aptamer.biophysical chemistry,126(1-3),165-175. |
| MLA | Bishop GR,et al."Energetic basis of molecular recognition in a DNA aptamer".biophysical chemistry 126.1-3(2007):165-175. |
入库方式： OAI收割
来源：长春应用化学研究所
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