Evaluation of different culture conditions for high-level soluble expression of human cyclin A(2) with pET vector in BL21 (DE3) and spectroscopic characterization of its inclusion body structure
文献类型：期刊论文
| 作者 | Wang XH ; Fu ML ; Ren JS ; Qu XG |
| 刊名 | protein expression and purification
 |
| 出版日期 | 2007 |
| 卷号 | 56期号:1页码:27-34 |
| 关键词 | COOMASSIE BRILLIANT BLUE ESCHERICHIA-COLI RECOMBINANT PROTEINS RNA-POLYMERASE BODIES PROMOTER G-250 |
| ISSN号 | 1046-5928 |
| 通讯作者 | qu xg |
| 中文摘要 | in this paper, we evaluated various parameters of culture condition affecting high-level soluble expression of human cyclin a, in escherichia coli bl21(de3), and demonstrated that the highest protein yield was obtained using tb(no glycerol) + 0.5% glucose medium at 25 degrees c. by single immobilized metal ion affinity chromatography, we got highly purified human cyclin a(2) with a yield ranged from 20 to 30 mg/l. by amyloid-diagnostic dye tht binding and fourier transform infrared spectroscopy, we observed a significant decrease in alpha-helix content and an increase in beta-sheet structure in cyclin a(2) inclusion body in comparison to its native protein, and confirmed the resemblance of the internal organization of cyclin a(2) inclusion body and amyloid fibrils. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000250639100004 |
| 公开日期 | 2010-07-13 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/14117]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Wang XH,Fu ML,Ren JS,et al. Evaluation of different culture conditions for high-level soluble expression of human cyclin A(2) with pET vector in BL21 (DE3) and spectroscopic characterization of its inclusion body structure[J]. protein expression and purification,2007,56(1):27-34. |
| APA | Wang XH,Fu ML,Ren JS,&Qu XG.(2007).Evaluation of different culture conditions for high-level soluble expression of human cyclin A(2) with pET vector in BL21 (DE3) and spectroscopic characterization of its inclusion body structure.protein expression and purification,56(1),27-34. |
| MLA | Wang XH,et al."Evaluation of different culture conditions for high-level soluble expression of human cyclin A(2) with pET vector in BL21 (DE3) and spectroscopic characterization of its inclusion body structure".protein expression and purification 56.1(2007):27-34. |
入库方式： OAI收割
来源：长春应用化学研究所
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