Poly(L-lysine)-graft-chitosan copolymers: Synthesis, characterization, and gene transfection effect
文献类型：期刊论文
| 作者 | Yu HJ ; Chen XS ; Lu TC ; Sun J ; Tian HY ; Hu J ; Wang Y ; Zhang PB ; Jing XB |
| 刊名 | biomacromolecules
 |
| 出版日期 | 2007 |
| 卷号 | 8期号:5页码:1425-1435 |
| 关键词 | CHITOSAN-DNA NANOPARTICLES POLYETHYLENIMINE IN-VITRO DELIVERY-SYSTEM SIDE-CHAIN VIVO DERIVATIVES EFFICIENCY PROTECTION POLYMERS CARRIERS |
| ISSN号 | 1525-7797 |
| 通讯作者 | jing xb |
| 中文摘要 | polypeptide/polysaccharide graft copolymers poly(l-lysine)-graft-chitosan (pll-g-chi) were prepared by ring-opening polymerization (rop) of epsilon-benzoxycarbonyl l-lysine n-carboxyanhydrides (z-l-lysine nca) in the presence of 6-o-triphenylmethyl chitosan. the pll-g-chi copolymers were thoroughly characterized by h-1 nmr, c-13 nmr, fourier transform infrared (ft-ir), and gel permeation chromatography (gpc). the number-average degree of polymerization of pll grafted onto the chitosan backbone could be adjusted by controlling the feed ratio of nca to 6-o-triphenylmethyl chitosan. the particle size of the complexes formed from the copolymer and calf thymus dna was measured by dynamic light scattering (dls). it was found in the range of 120 similar to 340 nm. the gel retardation electrophoresis showed that the pll-g-chi copolymers possessed better plasmid dna-binding ability than chitosan. the gene transfection effect in hek 293t cells of the copolymers was evaluated, and the results showed that the gene transfection ability of the copolymer was better than that of chitosan and was dependent on the pll grafting ratio. the pll-g-chi copolymers could be used as effective gene delivery vectors. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000246413600009 |
| 公开日期 | 2010-07-13 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/14419]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Yu HJ,Chen XS,Lu TC,et al. Poly(L-lysine)-graft-chitosan copolymers: Synthesis, characterization, and gene transfection effect[J]. biomacromolecules,2007,8(5):1425-1435. |
| APA | Yu HJ.,Chen XS.,Lu TC.,Sun J.,Tian HY.,...&Jing XB.(2007).Poly(L-lysine)-graft-chitosan copolymers: Synthesis, characterization, and gene transfection effect.biomacromolecules,8(5),1425-1435. |
| MLA | Yu HJ,et al."Poly(L-lysine)-graft-chitosan copolymers: Synthesis, characterization, and gene transfection effect".biomacromolecules 8.5(2007):1425-1435. |
入库方式： OAI收割
来源：长春应用化学研究所
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